FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_128.35100000056e5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_128.35100000056e5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201836
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA16670.8259180720981391No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG9220.45680651618145424No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6860.33987990249509503No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT6800.3369071919776452No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT6210.3076755385560554No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT5370.2660575913117581No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5150.2551576527477754No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC5110.2531758457361422No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCACATCC5040.24970768346578412No Hit
GTCTATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG4880.24178045541925125No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4830.23930319665470975No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4690.2323668721139935No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4580.22691690283200222No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4540.22493509582036902No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC4070.20164886343367883No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG4070.20164886343367883No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC4010.198676152916229No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3830.1897580213638796No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3770.18678531084642977No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3530.17489446877663054No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3420.1694444994946392No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3390.16795814423591432No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT3300.1634990784597396No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3290.16300362670683127No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA3210.15904001268356488No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3200.15854456093065658No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA3020.14962642937830714No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT3020.14962642937830714No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2980.14764462236667394No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2960.14665371886085735No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2910.14417646009631582No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCTATAGACGCATTTGAAAAAAAGACG2840.1407082978259577No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC2830.1402128460730494No Hit
TCCCAGCACAGGTCTCACAGGCAGATGGTGGCAACAACCAATCCATTAAT2490.12336748647416715No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2390.11841296894508414No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2390.11841296894508414No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2370.11742206543926752No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC2360.11692661368635923No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2360.11692661368635923No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2350.11643116193345092No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC2300.1139539031689094No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2300.1139539031689094No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT2280.1129629996630928No Hit
GTGTTATCATTCCATTCAAGCCCTCCGATGAGGACCCCAATTGCATTTTT2240.1109811926514596No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2150.10652212687528488No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2150.10652212687528488No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGGCTTGAA2150.10652212687528488No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA2100.10404486811074337No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2090.10354941635783509No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2020.10008125408747696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTTT150.0049639465163.33333245
GCAGGGT2850.0150.43869
GCAGGGG1700.0144.117659
CGAAAGC2250.0136.111113
AGCAAAA10500.0126.02
GCAAAAG10650.0124.225363
AGCAGGG5600.0122.58
GCGAAAG2800.0109.3752
CAAAAGC12350.0107.125514
AAGCAGG15500.098.07
AGGGTGT1900.096.7105266
ACAAGGT1400.096.258
TACTGAT407.549723E-491.875017
GTACTGA407.549723E-491.875016
GAGCAAA15400.085.909091
AGCGAAA3850.079.545451
AAAAGCA16950.078.053095
GCAGGTA6950.077.5539559
GAGCGAA2650.073.9622651
AAGGGTG2700.072.592595
Produced by FastQC (version 0.11.2)