FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_127.35100000056e09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_127.35100000056e09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152503
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8350.5475302125204095No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4180.27409296866291155No Hit
CTAATAGATGCTCTATTGGGAGATCCTCAGTGTGATGGCTTTCAAAATAA3570.23409375553267806No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3280.2150777361756818No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2830.1855701199320669No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2610.1711441742129663No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2390.15671822849386569No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2330.1527838796613837No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2290.15016098043972906No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2200.14425945719100608No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCTGCTGT2060.13507930991521477No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCT2050.13442358510980112No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2030.1331121354989738No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2030.1331121354989738No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1970.1291777866664918No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1900.12458771302859616No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1820.11934191458528684No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1790.11737474016904587No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1780.1167190153636322No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1740.11409611614197754No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1730.11344039133656388No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1710.11212894172573655No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1620.10622741847701356No Hit
GCATCTACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1620.10622741847701356No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1620.10622741847701356No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1620.10622741847701356No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1610.1055716936715999No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1580.10360451925535892No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1580.10360451925535892No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1580.10360451925535892No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1570.10294879444994526No Hit
ATGTTTGGAGATTGCCTGTAAGAACCTCTTCTTCTTTTTTGACTGACGAC1570.10294879444994526No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1570.10294879444994526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTT100.00147495245.000024
AGGCCCG100.00147495245.000028
GCAGGGT750.0212.333339
AGGGTGT204.77822E-5183.750026
GCAGGGG1300.0169.61549
ACGTTCT150.0049627637163.33333245
CCTAGAT150.0049627637163.333336
AGCAGGG3650.0104.04118
AAGCAGG6900.0101.195657
GCAAAAG6250.098.03
AGCAAAA7400.084.4256742
CAAAAGC7650.081.6666644
AGCAGGT2950.078.898318
CGAAAGC1101.5716068E-977.9545443
TTTAAAC500.001830361373.53
TACGGAT500.001830361373.52
CGGATAA500.001830361373.54
ACGGATA500.001830361373.53
TCTATGC852.3816847E-672.058823
ATATTCA707.906622E-570.04
Produced by FastQC (version 0.11.2)