FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_124.35100000056dd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_124.35100000056dd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220696
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT32761.4843948236488202No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT29981.3584296951462647No Hit
GTTCTTAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTG16400.7431036357704716No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT13530.6130604995106391No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG13370.6058107079421466No Hit
CCTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCTGGTACATCTGTT13320.6035451480769928No Hit
GCAATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAG12030.5450937035560228No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTGACCGAGGTCGAA11900.5392032479066227No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAA11320.5129227534708377No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC10210.4626273244644217No Hit
GCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGAACCAAGACGCA10130.4590024286801754No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA9910.44903396527349837No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA8080.3661144742088665No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC7840.3552397868561279No Hit
GTTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGG7800.3534273389640048No Hit
GTTAAAGCACAGATTTGAAATAAATCAGAAGCATGGCCCATTGGGGAGTC7330.33213107623155835No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA7300.33077174031246603No Hit
GCAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTT7130.32306883677094284No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6150.27866386341392685No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6040.27367963171058834No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5980.27096095987240365No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5980.27096095987240365No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT5770.2614456084387574No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA5660.25646137673541886No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT5620.25464892884329576No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5590.25328959292420344No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGAA5460.24739913727480334No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA5270.23879000978721862No Hit
CTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAGTC5110.2315402182187262No Hit
GCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTTT4660.2111501794323413No Hit
ATTTTAATGCATGTGTCAAGAAGGAGTTGAACCAAGACGCATTGAGCAAA4610.20888461956718746No Hit
CTTAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTGGAG4580.2075252836480951No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4480.20299416391778738No Hit
ATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAAC4380.19846304418747962No Hit
ATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAGTAG4380.19846304418747962No Hit
CCTATATGAACTACTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCAGT4270.1934788124841411No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4250.19257258853807951No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4250.19257258853807951No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4220.1912132526189872No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC4170.18894769275383333No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3970.1798854532932178No Hit
ATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAG3940.1785261173741255No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3940.1785261173741255No Hit
GAGTACAAATTTGCAGCAATATGCACTCACTTGGAGGTGTGTTTCATGTA3850.1744481096168485No Hit
CTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA3830.17354188567078696No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3800.17218254975169464No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAG3790.17172943777866387No Hit
GTAGTTCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATG3770.17082321383260232No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT3760.17037010185957155No Hit
CTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTG3720.16855765396744843No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3570.1617609743719868No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3490.15813607858774062No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3430.15541740674955595No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3390.15360495885743283No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC3300.14952695110015587No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3290.1490738391271251No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA3170.1436364954507558No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA3140.14227715953166348No Hit
ATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGA2960.1341211440171095No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATTCA2960.1341211440171095No Hit
CAGGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGG2940.13321492007104796No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2930.1327618080980172No Hit
TCTGAATATTGCTGCAAATTTGTTGGTTTCAATTTTCAGATCCTCCCCAT2920.1323086961249864No Hit
CAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGT2870.13004313625983255No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2850.12913691231377097No Hit
TCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAGT2810.12732446442164788No Hit
GTCAAGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCATT2780.12596512850255556No Hit
GACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCAC2760.125058904556494No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACACTATG2750.12460579258346323No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2740.12415268061043246No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2690.12188712074527858No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT2690.12188712074527858No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2680.12143400877224779No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2670.12098089679921703No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2650.12007467285315547No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2640.11962156088012468No Hit
GAATACATGAAACACACCTCCAAGTGAGTGCATATTGCTGCAAATTTGTT2490.11282488128466306No Hit
GAAATTTGGCCTATGGCACTCCTTAGTAACATATCTCCTATCTCAAGGAC2450.11101243339253995No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTT2400.1087468735273861No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2390.10829376155435531No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2390.10829376155435531No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2370.10738753760829375No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2360.106934425635263No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTTCAAATCTG2360.106934425635263No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTGACCGAGGTCGAAAC2360.106934425635263No Hit
AAGCACAACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG2340.10602820168920143No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2330.10557508971617065No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2330.10557508971617065No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2270.10285641787798601No Hit
ATTGGGGAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTCTG2270.10285641787798601No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCTAAGAG2240.10149708195889369No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2230.1010439699858629No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2210.10013774603980134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG650.0169.615379
GTTTCGG150.004964259163.33333245
CCAGAGT150.004964259163.333331
GAGCGAA3050.0160.655751
GCAGGGT3250.0147.09
GCAAAAG17100.0144.707613
AACCGAG601.2732926E-11142.916675
AAGCAGG20600.0142.718447
CAAAAGC17800.0139.705064
CGAAAGC3700.0132.432434
TTCTAAC652.3646862E-11131.923071
AGCAGGG4700.0127.712778
AGCAGGT11250.0124.133348
GCGAAAG3950.0124.050643
GGGTATT302.404702E-4122.57
CTAACCG704.3655746E-11122.53
AGCGAAA4100.0119.512192
GCAGGTA10650.0115.023479
TAACCGA757.6397555E-11114.3333364
GGTAGTT551.7876664E-7111.363637
Produced by FastQC (version 0.11.2)