FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_123.35100000056d5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_123.35100000056d5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203793
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC29221.4338078344202205No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA20981.0294759878896724No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC14210.6972761576697923No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13720.673232152232903No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA13300.6526230047155692No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG10390.5098310540597567No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC9140.44849430549626335No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT7510.36851118536946803No Hit
GCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT7360.3611507755418489No Hit
TTGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTG7330.35967869357632504No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7180.35231828374870583No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG6990.3429950979670548No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6860.3366160761164515No Hit
GCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACT6190.30373957888641906No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG5960.29245361715073626No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5710.2801862674380376No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG5630.27626071552997405No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT5620.2757700215414661No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5130.2517260161045767No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA4840.23749589043784627No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC4490.22032160084006813No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA4460.2188495188745443No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4380.2149239669664807No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4260.20903563910438533No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC4130.20265661725378203No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4000.1962775954031787No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3900.19137065551809926No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT3860.18940787956406746No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3820.18744510361003566No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3800.18646371563301978No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA3690.18106608175943237No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3670.18008469378241648No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3530.17321497794330523No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3480.17076150800076548No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG3370.16536387412717807No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3370.16536387412717807No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3340.16389179216165423No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3290.1614383222191145No Hit
GCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC3230.15849415828806682No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTC3200.15702207632254297No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3120.1530965244144794No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG3120.1530965244144794No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3090.15162444244895557No Hit
ATATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTT3080.1511337484604476No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3020.14818958452939993No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2910.14279195065581252No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2770.13592223481670127No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2670.13101529493162178No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2670.13101529493162178No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2660.13052460094311386No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2630.12905251897759001No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2620.12856182498908206No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2600.12758043701206617No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2580.12659904903505026No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2540.12463627308101849No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2490.12218280313847875No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2460.12071072117295491No Hit
CATTGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTC2460.12071072117295491No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2410.11825725123041518No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTT2380.11678516926489134No Hit
ATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTC2380.11678516926489134No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2350.1153130872993675No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2330.1143316993223516No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2320.11384100533384366No Hit
CCATATGGCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2310.11335031134533571No Hit
GAAATATCCAATCAACAATTCTTGGAAAAGATGCCGGTACTTTAATTGAA2250.11040614741428803No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2220.10893406544876419No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2180.10697128949473239No Hit
ATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC2170.10648059550622446No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2140.10500851354070062No Hit
GGCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAA2130.10451781955219266No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT2110.10353643157517678No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2110.10353643157517678No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC2070.10157365562114498No Hit
GGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCT2070.10157365562114498No Hit
TGGCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAA2060.10108296163263704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAG100.0014753131245.00002245
CCGGAGG304.789399E-9204.16667245
GGTACCC150.0049639815163.333331
AGTAGGG150.0049639815163.333332
AAAGCGG150.0049639815163.333336
GCAGGGG1400.0148.759
GCAGGGT4000.0147.09
GCAAAAG15700.0146.687913
CAAAAGC17200.0133.895364
GAGCGAA2500.0132.31
AAGCAGG19900.0131.733677
GCAGGTC8200.0131.463419
CGAAAGC3550.0131.126753
AGCAAAA17750.0129.746482
AGCAGGG6300.0126.388898
GAGCAAA18400.0125.828811
AGCAGGT13550.0125.66428
GCGAAAG3800.0122.52
CATAATT1450.0109.827593
AGCGAAA4350.0107.01151
Produced by FastQC (version 0.11.2)