FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_117.35100000056cd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_117.35100000056cd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168989
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10000.5917544928959872No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC7570.4479581511222624No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA7370.43612306126434264No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6050.35801146820207236No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC5780.3420340968938807No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT5770.3414423424009847No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT5620.3325660250075449No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5550.32842374355727294No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG5350.31658865369935324No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG5210.3083040907988094No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4940.2923267194906177No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4750.28108338412559397No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA4460.26392250383161037No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA4460.26392250383161037No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG4350.25741320440975446No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG4310.25504618643817056No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG4000.2367017971583949No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT3910.23137600672233105No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC3790.2242749528075792No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT3610.21362337193545142No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3470.20533880903490762No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3360.19882950961305174No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC3360.19882950961305174No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC3320.19646249164146778No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3250.19232021019119588No Hit
CTCTTAGACCAGTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC3110.18403564729065205No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG2890.17101704844694035No Hit
GACTTGAAGATGTCTTTGCTGGAAAAAACACAGATCTTGAGGCTCTCATG2800.16569125801087645No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2770.1639159945321885No Hit
TCCTAGTTCCAGCACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2620.15503967713874867No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC2600.15385616815295672No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2530.1497138867026848No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT2510.14853037771689281No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2510.14853037771689281No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA2480.14675511423820486No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2460.1455716052524129No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA2450.1449798507595169No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2430.1437963417737249No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2400.14202107829503696No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT2400.14202107829503696No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT2390.14142932380214096No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2300.13610353336607708No Hit
TTCCAGCACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2300.13610353336607708No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2270.1343282698873891No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT2260.13373651539449313No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2250.13314476090159716No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG2170.12841072495842926No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA2170.12841072495842926No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT2160.12781897046553325No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA2140.12663546147974128No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2140.12663546147974128No Hit
TCTTAGACCAGTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT2130.1260437069868453No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA2130.1260437069868453No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC2110.12486019800105333No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC2070.12249318002946936No Hit
TAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGA2050.1213096710436774No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2040.12071791655078142No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2040.12071791655078142No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA2030.12012616205788543No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2010.11894265307209345No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT2000.11835089857919745No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC2000.11835089857919745No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT1990.11775914408630146No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1990.11775914408630146No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC1970.1165756351005095No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1920.11361686263602956No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTGCTG1920.11361686263602956No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1890.1118415991573416No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1860.11006633567865365No Hit
CTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAGCTCTCAGTTA1820.1076993177070697No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1820.1076993177070697No Hit
GGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTGGTTGTTGCCA1820.1076993177070697No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC1820.1076993177070697No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA1810.10710756321417371No Hit
ATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCA1800.10651580872127772No Hit
GTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1800.10651580872127772No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT1790.10592405422838173No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG1780.10533229973548573No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG1730.10237352727100581No Hit
TTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAG1720.10178177277810982No Hit
AGTAATGACTGATGGAAATGCTACAGGAAAAGCTGATACTAAAATACTAT1710.10119001828521382No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1710.10119001828521382No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG1700.10059826379231783No Hit
GGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGCACTGGTCTAAGAG1700.10059826379231783No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1690.10000650929942186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG400.0214.375029
TCATATG302.403714E-4122.55
GCAAAAG4900.0117.500013
GCAGGGT1150.0117.173919
AGTACTA458.832136E-6108.888885
GTCGTAC354.4386892E-4105.000011
AAGCAGG6500.0103.653847
CGAAAGC953.6379788E-12103.157893
AGCAGGT4150.0100.361448
GCAGGTA3850.098.636379
AGCAGGG2300.095.869568
TTTAAAC407.5475767E-491.875013
CGTACTC407.5475767E-491.875013
GAGCGAA954.9294613E-1090.263151
CAAAAGC6600.089.090914
AGCAAAA7150.082.237762
AGGGTGT903.501009E-881.6666646
GCGAAAG1306.91216E-1175.384612
TACGGAT655.4786833E-575.384612
GTACTAA655.4786833E-575.384616
Produced by FastQC (version 0.11.2)