FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_115.35100000056c5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_115.35100000056c5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201075
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA18030.8966803431555389No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA9120.453562103692652No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC8500.42272783787144097No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC7850.39040159144597786No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT7390.3675245555141116No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC6670.3317170210120602No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6590.3277384060673878No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG6110.3038667163993535No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT5990.29789879398234487No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG5890.2929255253015044No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5810.288946910356832No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA5220.2596046251398732No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG5130.25512868332711675No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG5090.2531393758547806No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC4950.2461767997016039No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG4930.24518214596543578No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG4840.24070620415267935No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4670.23225164739525053No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA4620.22976501305483027No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG4600.22877035931866221No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT4220.20987193833146836No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC4160.20688797712296406No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3890.19346015168469477No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA3650.1815243068506776No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC3570.17754569190600522No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3450.17157776948899664No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC3450.17157776948899664No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC3400.1690911351485764No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT3270.16262588586348378No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3260.1621285589953997No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3150.1566579634464752No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3140.15616063657839116No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3060.15218202163371877No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT3060.15218202163371877No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG3040.15118736789755066No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3020.15019271416138255No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2930.14571677234862615No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT2910.14472211861245804No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG2810.13974884993161754No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2800.1392515230635335No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT2800.1392515230635335No Hit
CCCCCATTCGTTTCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTT2780.1382568693273654No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA2750.13676488872311326No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA2740.13626756185502922No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2660.13228894691035684No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT2660.13228894691035684No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2650.1317916200422728No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC2610.1298023125699366No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2510.12482904388909612No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2480.12333706328484395No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2450.12184508268059183No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC2430.12085042894442373No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA2420.12035310207633967No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2400.11935844834017158No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA2390.11886112147208752No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2340.11637448713166729No Hit
TAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGA2320.11537983339549919No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2320.11537983339549919No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA2280.113390525923163No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA2280.113390525923163No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTGGGG2270.11289319905507895No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC2250.11189854531891084No Hit
AGTAATGACTGATGGAAATGCTACAGGAAAAGCTGATACTAAAATACTAT2240.1114012184508268No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT2230.11090389158274275No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2220.11040656471465872No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT2220.11040656471465872No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA2220.11040656471465872No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT2210.10990923784657466No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC2210.10990923784657466No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT2190.10891458411040657No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT2180.10841725724232251No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG2160.10742260350615442No Hit
ACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGAC2140.10642794976998633No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA2140.10642794976998633No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA2080.10344398856148203No Hit
TTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAG2080.10344398856148203No Hit
GGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTGGTTGTTGCCA2060.10244933482531395No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC2030.10095735422106179No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCTGGTCACT2020.10046002735297774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTAA204.7804722E-5183.750025
TAGAAGT150.0049639326163.333334
GTATTAA251.1633091E-4146.999986
CAAGCTA251.1633091E-4146.999984
GCTAAAT251.1633091E-4146.999987
GCAGGGT1300.0122.59
GCAGGGG1000.0122.499999
GCAAAAG7300.0107.397263
GAGCGAA1850.0105.9459461
GCAGGTA5900.0103.813569
CGAAAGC1900.0103.157894
AGGGTGT953.6379788E-12103.157896
AGCAGGT6200.0102.741938
AAGCAGG9350.0102.192527
AAGGGTG1101.6370905E-1189.090915
TAGAAAC1900.083.815792
AGCAAAA9400.083.404262
ATAGGGA450.00120537981.666676
AATAGGG450.00120537981.666675
ACAAGGT1500.081.666677
Produced by FastQC (version 0.11.2)