FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_106.35100000056e30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_106.35100000056e30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161378
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9340.5787653831377263No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT8710.5397266046177298No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAC7360.45607207921773724No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6630.41083666918663014No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5500.34081473311108085No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5440.3370967542044145No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT5290.32780180693774863No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4520.2800877443021973No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3780.23423267111997917No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3470.21502311343553643No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3370.20882648192442588No Hit
CTAGTAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTAC3190.19767254520442687No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2990.18527928218220574No Hit
ATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2930.1815613032755394No Hit
TTGTCTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTTTCAGG2510.15553545092887505No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2420.14995848256887556No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2330.14438151420887604No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAC2250.1394242089999876No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2180.13508656694221022No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2130.13198825118665494No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2050.12703094597776649No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2040.12641128282665542No Hit
TTTTTAAACTATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCG2010.12455229337332227No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1990.12331296707110014No Hit
GTTCCCTGTCCAAGGGCAAATTGATAACACTTTTCAGGATCGCATGACAC1990.12331296707110014No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1980.12269330391998909No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1970.12207364076887803No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT1920.11897532501332275No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1910.11835566186221169No Hit
ACTCCTAGATCTCCTTATGCAAGCAGAACTTGTTCCGTTTTGAGTGACTC1890.11711633555998958No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1860.11525734610665643No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1840.11401801980443431No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTATTCATATCAAAA1840.11401801980443431No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1830.11339835665332325No Hit
CTACTCCTAGATCTCCTTATGCAAGCAGAACTTGTTCCGTTTTGAGTGAC1830.11339835665332325No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT1800.1115393671999901No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT1730.1072017251422127No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1730.1072017251422127No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1730.1072017251422127No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1720.10658206199110162No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC1720.10658206199110162No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1700.10534273568887952No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1690.10472307253776847No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTACGG1640.10162475678221318No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG151.5169577E-5245.01
TGTGTAT204.7787325E-5183.750029
CGTCAGA204.7787325E-5183.750022
GCAGGGG1000.0159.259
GTAGCCG402.6697307E-8153.1254
GCAGGGT2950.0128.72889
GCTTATA302.4035157E-4122.52
AGCAAAA9500.0121.2105262
CGAAAGC2250.0119.777784
CAAAAGC10800.0106.620374
GAGCGAA2600.0103.653841
AAGCAGG13900.0102.230227
GTCGTAC602.9989314E-7102.0833361
GAGCAAA11450.0101.637561
ATCTAGT852.0372681E-10100.8823551
AGCAGGG5350.0100.747668
AGTACTA1100.0100.227275
TACCCGC501.4904583E-598.02
GCAAAAG12100.096.177683
AGCAGGT8550.094.56148
Produced by FastQC (version 0.11.2)