FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_p2_3.35200000080c4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_p2_3.35200000080c4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35344
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGT100.0014701637244.999987
GTCTATT500.001818678273.52
TAGGATT550.002654107866.818184
TCTATGC600.003746836461.253
ATTAATG700.00689662452.5000043
GTGTAAA750.00905903949.01
ATAAGTA350.00719410228.00000285-89