FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_p1_3.35200000080c3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_p1_3.35200000080c3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130902
Sequences flagged as poor quality0
Sequence length251
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTAC302.4024892E-4122.57
CATTTTA354.4364322E-4105.06
TTTTACC354.4364322E-4105.08
TTTACCA407.5437466E-491.875019
GTTATCA407.5437466E-491.875011
TTATCAT450.001204433981.6666642
TCTCTTT801.6598933E-676.5625245
TCATTTT500.001829779173.55
TTAGAGC150.00912992149.095-99
CTATAAG150.00912992149.035-39
GCTATAA150.00912992149.035-39
TTAGTGA150.00912992149.0160-164
ATCATTT750.00911372749.04
TATAAGG150.00912992149.035-39
AATTTAC250.001386454639.265-69
AGTTTAT250.001386454639.2130-134
GTAAGAG250.001386454639.2240-244
AAATTTA300.003391944632.66666865-69
ACAAATT350.00720813728.00000270-74
CCTTGTT1750.007187415428.0000029
Produced by FastQC (version 0.11.2)