FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_b3_2.35200000080c65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_b3_2.35200000080c65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107869
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA38600.018.0893781
CGGTGGG15050.017.906977245
GGATAGG15100.017.847683245
GTCCTAT12550.017.5697211
GTATATA41100.016.9890521
ATAGGGG8001.1261545E-716.843753
TCTCGGG16150.016.687305245
CATAGGG7701.3961835E-615.909092
GTATAGA26450.015.7466921
GTATTAG19050.015.433071
CTATACT25900.015.1351364
TACACCG12954.3655746E-1115.1351365
GTATAAT42200.014.80450251
CTAGGCC5000.004295155414.7000013
GTATGAG20900.014.653111
ATAGGGC9205.506445E-714.646748
GTCTAGG9205.506445E-714.646741
TAATAGT37250.014.4697994
TCCTATG15353.6379788E-1214.3648212
GGACCGC6008.7139104E-414.2916676