FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_b2_3.35200000080c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_b2_3.35200000080c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670781
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGG10800.029.490742245
GTATAGG9350.023.5828881
CCCTAGG3752.386454E-522.8666671
GTATATA32950.021.934751
GTGTAAG15750.019.4444451
CTATACT18500.018.540544
GTACTAG6854.1069143E-717.8832111
TATAGGA18250.017.4520552
GTCTAGA10653.6379788E-1117.2535211
ATAGGAC11557.2759576E-1216.9696963
GTACTGG14700.016.6666661
CCGTGTG9602.9758667E-916.5885419
GTATAGA20550.016.094891
ACACCGC10909.422365E-1015.7339456
ACCGTGT9555.0087692E-815.3926718
TATCCCG10358.059942E-915.3864735
CGAAGGG12002.237357E-1015.3125245
TATACTG20000.015.3124995
CCCTATA9756.459413E-815.0769232
TATATAG26350.014.8766592
Produced by FastQC (version 0.11.2)