FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_b1_3.35200000080c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_b1_3.35200000080c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919997
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG11500.022.3695641
GTCCTAA11750.020.8510631
TATACTG26300.019.5627375
GTATAAG20350.018.6609341
GTATATG33900.018.0678461
GTATAAT36200.017.5966841
GTATAGA20250.017.5432111
GTCTAGC7156.4366395E-717.1328681
ACAGTCC13100.016.832068
TATAAGG22700.016.7290742
GTATATA38900.015.74550151
GTGTAGG13707.2759576E-1215.2007281
ACAGGGG9706.066148E-815.154648
TATACCG13407.4578566E-1114.6268655
TACACTG19450.014.4858625
TCTCGGG12805.9117156E-1014.35547245
TAGACTG12906.6393113E-1014.2441855
CGATACC42300.014.190308245
CTGTCCG8755.2677224E-614.09
CTGTGCA26350.013.9468689
Produced by FastQC (version 0.11.2)