FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n02_b1_2.35200000080c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n02_b1_2.35200000080c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105400
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG11650.018.9270381
CTGTGCG11500.018.1086969
CCTAGGG4751.4714245E-418.0526313
ACCGCGT5652.8160704E-517.3451338
CCTAGAC10153.4197E-1016.8965513
TAGACCG6555.3042822E-616.832065
GTATTAA33000.016.7045441
GTATTAG20800.016.4903851
TCTCGGG18800.016.289894245
ACTGTGT22000.015.5909098
TATTAGG19250.015.2727282
TATACTG25900.015.1351365
TATAGGG10601.105218E-815.0235842
GTATAAT38300.014.7127931
GACGAAA41100.014.604623245
ACCGCGC5907.6727645E-414.5338988
GTATTAT40750.014.4294471
TCTAGAC14602.0008883E-1114.2636993
CCTATAC14852.5465852E-1114.0235693
ACTGTGC20450.013.7775068
Produced by FastQC (version 0.11.2)