FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_p2_3.35100000080c4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_p2_3.35100000080c4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35344
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1340.37913082842915347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGAG100.0014701637244.999989
ACAGCGA150.0049467077163.333338
ATAGACC407.49778E-491.874993
CTGAAAG1004.5612725E-449.09
ATTACTA652.3724706E-422.615385230-234
GTTGTTG550.002394211722.2727261
Produced by FastQC (version 0.11.2)