FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_p1_3.35100000080c32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_p1_3.35100000080c32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130902
Sequences flagged as poor quality0
Sequence length251
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1510.11535347053520954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCT150.0049619656163.333333
TAAAGAC302.4024892E-4122.55
CTCTAAA302.4024892E-4122.52
ACTCTAA407.5437466E-491.875011
CTAAAGA450.001204433981.6666644
TCTAAAG450.001204433981.6666643
TAAATTT351.7096804E-435.000004225-229
GTGTTGC1700.006238736728.8235281
ACCAGTT1850.00942546226.4864862
GTGCTGT4302.63119E-622.7906991
GTCCAGG6155.3933036E-921.910571
TCCAGGG3100.004788230619.7580642
GTCCTGG4408.106227E-519.4886341
GTTCTGC3350.007494277318.2835831
CAACAGA700.00968604617.500002240-244
CTCATCT700.00968604617.500002235-239
GGCGAAA4350.00168547816.896553245
AATATAA950.003050186215.473684230-234
AACAATA1309.3263196E-515.076923240-244
GTGTTGA1500.00465572211.433333125-129
Produced by FastQC (version 0.11.2)