FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_p1_2.35100000080c75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_p1_2.35100000080c75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659
Sequences flagged as poor quality0
Sequence length251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT260.9778112072207596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC40.15043249341857842No Hit
GGTTTCACCACTGCACCTGTTGGGGGCTGTATTTGGAATAGGGCCGAATT30.11282437006393381No Hit
AAAAGTATCAGGACTAACAAATCATGAAATCTTGTTCGTCATATTCAAAC30.11282437006393381No Hit
GATTAAACATGACTGAATCTTTCGCTCAACTCTTTGAAGAATCCCTGAAA30.11282437006393381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGG100.0013943573245.000027
Produced by FastQC (version 0.11.2)