FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_b3_2.35100000080c68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_b3_2.35100000080c68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107869
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA25250.022.3168321
GTATTAG19450.019.524421
GTATATA41200.018.4344651
GTATAAT36500.018.1232851
TATACAG29650.017.76565
TATACTG27450.016.9581055
TACCGTG11709.094947E-1216.7521367
CTGTCCT19850.016.045349
GTATTAA38550.015.8884561
TAGACAG19400.015.7860825
TGGACCG10408.594725E-915.3124995
GTATTAT43300.014.9942271
GTATAGG11451.893568E-914.9781661
GGACCGG7552.0398911E-514.602656
TTATACT42000.014.5833334
ACTGTGC18550.014.5283028
GGCGAAA35650.014.431977245
TACACCG12755.5661076E-1014.4117655
TACACTG20450.014.3765295
GTATGAG21500.014.2441861
Produced by FastQC (version 0.11.2)