FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_b2_3.35100000080c25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_b2_3.35100000080c25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670781
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG9200.023.96739245
TAGACTG10350.022.4879235
GTATAGG9103.6379788E-1220.1923071
GTATATA33500.018.6492541
ACCGTCC6602.7796705E-718.5606068
CCCTAGG3450.00892150317.7536221
TAGAGTG11455.456968E-1217.1179055
GTCTACG5853.7946495E-516.7521361
CTATAGG8801.830449E-816.7045441
CCTAGGA6656.125103E-616.5789492
ACCGTGG9703.416062E-916.4175268
GTATAAG18950.016.160951
CTTAGGG6105.4299933E-516.0655732
TATACTG22950.016.0130735
GTATTAG14600.015.9417811
ACTGTGC15400.015.9090928
GTATAAA35600.015.8286511
ACCGTGC8602.563247E-715.6686048
TCTAGGC7101.1403687E-515.528173
TACACCG11051.1441443E-915.5203625
Produced by FastQC (version 0.11.2)