FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_b1_3.35100000080c18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_b1_3.35100000080c18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919997
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG4705.7576017E-620.8510631
GTATAGG9400.020.8510631
GTATAGT15400.020.681821
GTATAGA22850.019.2997821
TACACCG12750.019.2156875
GTCTAGG8258.247298E-917.8181821
ACTGTGC19450.017.6349628
GTATAAT33850.017.3707541
GTATATG31350.017.1929821
TAGGAGT12201.8189894E-1217.0696724
GTATATA37450.016.6822431
TATACTG26850.016.424585
TATACAG26300.015.8365025
ACTGTGT19400.015.7860838
GTATTAC22750.015.6153841
GTATTGG22100.015.5203621
GTATTAA33150.015.5203611
GTATTAT38250.015.3725491
TTATACT35400.014.8799434
GGGGGCT4950.0040174314.8484866