FastQCFastQC Report
Thu 16 Mar 2017
000000000-ANUJL_l01n01_b1_2.35100000080c5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01n01_b1_2.35100000080c5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105400
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG24550.021.4562115
CTAAGGG9900.019.79798245
GTATAAG23600.019.7245771
TAGGACC8153.3651304E-1019.5398794
CCCTAGG5101.166306E-519.2156871
GTATTAA33950.018.7628861
TAGGTCG4801.5942295E-417.8645845
GGCGAAA35700.016.813725245
TATACTG27500.016.4818175
GTATAGA21750.016.3333341
CCTAGGG4550.002291648416.1538473
GTAGGAC6858.118846E-616.0948893
GCCCTAA9152.9611329E-816.0655751
GTATATA38400.015.95052051
TAGGGGT7009.971422E-615.7499994
TAGGATA15800.015.506334
TCTATAC28500.015.4736833
GTACTAG9555.010952E-815.392671
GTATTAT40050.015.2933831
GTGTAGG10551.03846105E-815.0947871
Produced by FastQC (version 0.11.2)