FastQCFastQC Report
Tue 14 Sep 2021
000000000-ANUJL_l01_n01_P2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANUJL_l01_n01_P2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38524
Sequences flagged as poor quality0
Sequence length251
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1350.3504309002180459No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG400.10383137784238396RNA PCR Primer, Index 46 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)