FastQCFastQC Report
Mon 13 Feb 2017
000000000-ANFV3_l01n03.3330000005404e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANFV3_l01n03.3330000005404e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949391
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG7607438.500500201633832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG5330015.955723691142783No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG2855033.1901947294514232No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2645242.955776543901144No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA2495592.7885584616875048No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2489612.7818764427657703No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA2401302.6831993372509926No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1379951.5419484968306783No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1255581.4029781467811608No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1229391.3737135856506884No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1155731.291406309099692No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA1103501.2330447960090245No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1018511.1380774401297251No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA991071.1074161359136057No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA946001.0570551672175235No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA921671.0298689597984936No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA877510.9805248200687622No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATAACTTGGTA849220.9489137305544031No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG824180.9209341730627257No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG754850.8434652145604097No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA746750.8344143193654183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA681550.7615602000180794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA608350.6797669249226009No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG564430.6306909598653138No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG528740.5908111512839254No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA525800.587526011546484No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTACCTTAGTA511800.5718824889872395No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG502410.5613901549278605No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGCCCGGTG492160.5499368616255564No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG486230.5433107124272478No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA472700.5281923652682066No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG436140.4873404234992079No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA427270.477429134563458No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT408400.45634390094253346No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA381230.4259842932329138No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTATTGCCCCAGAA372080.415760133845979No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA364840.4076701978939125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG360640.4029771411261392No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGCCCAGAT336210.3756791942602575No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG278850.31158544754609563No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA278590.311294924984281No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG265300.2964447524976839No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG262530.2933495698198905No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA254820.2847344584676209No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA236640.2644202270299733No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG229320.25624089952042545No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTAACAGACCAGAG228090.254866504324149No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG222410.24851970374296975No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA220270.24612847958034237No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA199230.22261849996273486No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG190980.2133999955974658No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTTTAGTCCAGAA186780.20870693882969246No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATACCTTAGTA184560.20622632310958364No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG181440.20274005236780918No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAACAGTCCAGAG176110.19678433985061106No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTATCTGTCCAGAA170500.19051575688222808No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGGCCAGTA167860.18756583548534195No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT165230.18462708803314104No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA158230.17680532675351876No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG157350.1758220196212234No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG150550.16822373723530462No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG146340.16351950652284608No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA140620.15712801016292616No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCAAGTCCAGAA137030.15311656402094848No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA136390.15240143156109728No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA133110.14873637770436002No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATCTATTGTTAGTC128660.14376397231945726No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGCACAGGCCCAGAG116940.13066810914843255No Hit
CCTGTTTGATACCCACGCTTTCGTGCATCAGCGTCAGTTGTAGCCTAGTA112350.12553926853793737No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAATGTTGGTCCAGGT109820.12271226053258819No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT108910.12169543156623731No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG99040.11066674816196989No Hit
CCGGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCACCTTCTGTCCAGTA98020.10952700580408208No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTAAGCGTCAGTTGTCGTCCAGAA94020.10505742793001221No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA93270.1042193820786241No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA90610.10124711279236767No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTACAGACCAGGT90470.1010906775667752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC4431000.0244.621263
TTTGCTC3977200.0244.550325
CTGTTCG1617800.0244.51542
CCTGTTC1606050.0243.962661
TGTTTGA611750.0243.618323
CTCCCCA5448350.0243.232779
TTGATAC519550.0242.995886
GTTTGCT4497300.0241.897544
GCTCCCC5586100.0241.539528
CTGTTTG5112300.0241.017552
TGTTCGC1615250.0239.804983
TGTTCGA35550.0237.419163
TTTGATC100050.0236.919045
CCTGTTT5194150.0235.26211
CCCTCCG1670600.0233.55368245
CTACCCA661050.0232.806539
ATACCCA477300.0232.629389
CCCTATG112650.0231.18953245
GATACCC579750.0229.0478
CATACGT65200.0228.84204245
Produced by FastQC (version 0.11.2)