FastQCFastQC Report
Mon 13 Feb 2017
000000000-ANFV3_l01n02.33200000054041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANFV3_l01n02.33200000054041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949391
Sequences flagged as poor quality0
Sequence length12
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATGAGTG4963435.54610922687365No Hit
ATGATGACCCGT4249914.7488259256970675No Hit
GACTCGAATCGT3999444.468952133167496No Hit
TATGCACCAGTG3921574.381940625904042No Hit
CGCCAAATAACC3776594.21994077585838No Hit
TAACACCACATC3428353.830819326141857No Hit
GACACATTTCTG3359523.7539090648738No Hit
AAGATGGATCAG3312913.7018273086962004No Hit
TCAACAGCATCG3154883.5252454608363855No Hit
AGTTCCCGAGTA3128153.4953775066929134No Hit
CTTACACCAAGT2835523.1683943633706475No Hit
ACGGCATGGCAT2808573.1382805824441014No Hit
TACTTCGCTCGC2714133.032753848837312No Hit
CGTCCGAAATAC2358732.635631854726204No Hit
CGGACTACAACT2321932.5945117382847616No Hit
AGCCTAAGCACG2317172.5891929406146184No Hit
TCTGTTGCTCTC1964902.1955683911899704No Hit
ATTAGTTCGCGT1445351.6150260950717206No Hit
ACGATGCGACCA1135221.2684885485503985No Hit
TTATGCAGTCGT1024161.1443907188768487No Hit
CTAGATTTGCCA909051.0157674416058031No Hit
TGGCACCGATTA557170.622578676023877No Hit
GCAGGATAGATA401430.44855566149696663No Hit
GTCCGAAACACT385820.431113133843409No Hit
GTGGGATGTTTC352730.394138550880166No Hit
ATACCTTCGGTA299490.3346484693762961No Hit
CCATACATAGCT278350.31102675031183685No Hit
CTCGAGAGTACG275150.307451088012581No Hit
TTGATGCTATGC262950.29381887549666785No Hit
CCAAGTCTTACA243880.2725101629820398No Hit
ATGTCGAGAGAA238910.266956712473508No Hit
GGTCAGCTTAAC162920.18204590681086566No Hit
ATGGACCGAACC152460.17035796067017298No Hit
TAAACCGCGTGT150040.1676538660563607No Hit
ACCACATACATC136220.1522114745014493No Hit
TAGCGGATCACG135570.15148516809691295No Hit
AATGTCCGTGAC130620.1459540654777515No Hit
CTCTACCTCTAC118530.13244476635337532No Hit
ATGGTTGTTGGC116820.13053402181221047No Hit
TGACCTCCAAGA104200.11643250361952004No Hit
TCTACGGAGAGC99970.11170592501769114No Hit
TAAGGTAAGGTG97020.10840961133556462No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCA550.009159676.00000056
CTCTTGA550.009159676.00000052
ACTCGCA2400.06.00000052
CCTGGTG550.009159676.00000054
TGGTGCT600.00339611226.00000053
CCTGAGT550.009159676.00000055
GGCTCGG550.009159676.00000056
CCCGAGG550.009159676.00000055
CGGCATT1054.828926E-76.02
ATCAGGC650.0012617756.02
CTCCACG953.4287386E-66.05
CCGTCGT1054.828926E-76.06
TGCATTC806.537869E-56.04
ACGATGG2150.06.01
TCTACGG10000.06.01
CTACGGA10000.06.02
CCGAGTC5250.06.06
ACCAGGG909.149771E-66.06
GTTCCGA909.149771E-66.02
TCCCGGG704.6965477E-46.04
Produced by FastQC (version 0.11.2)