FastQCFastQC Report
Wed 10 Aug 2016
000000000-ANER6_l01n03.33300000067079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANER6_l01n03.33300000067079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406092
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTGTAACTATTAAGTTTTTTCAGACGCTGATTGCAACT10940845.9441406682092No Hit
CGTTGCTGAAAGTTGTATATAAATTGCGTTATAGCAAAGTATGACATTCT5681363.086673694774534No Hit
CGTTGTTGAAAGTTTTGAAGATTCGTATATCACTCGTCCTGCCACAGCGC4790742.60280128992075No Hit
CGTTGTTGAAAGTTGTAATTATTAATTTGTTACTGACGCTGATTGCAATT4202862.2834070371918167No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAATACATTCATAGACTT3077071.6717671518755854No Hit
CGTTGTTGAAAGTTGTATTAATTTAAAGGCATAAGCCCATTAATGACATT2608381.4171286332807638No Hit
CGTTGTTGAAAGTTTTGAAGATTCGTATATCACTCGTCCTGCCACAGTGC2175231.1817989391773116No Hit
CGTTGTTGAAAGTTTTGAAGATTTTAATTTTTGTTAAAAATTTTCATGAC2050221.113881208460764No Hit
CGTTGTTGAAAGTTTTGAAGATTTATTTGAAATTAATCAACAAATTGACA1995381.0840867251994613No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT1926701.0467729923331905No Hit
CGTTGTTGAAAGTTTTATTTTTTGTACATTATACAATAAATCAATTTAGG1830360.9944316262246218No Hit
CGTTGTTGAAAGTTTTGACTATTGTAATAATAAATCAAGTTTGACTGTAA1582940.8600087405843674No Hit
CGTTGTTGAAAGTTTTGAAGATTCGTGTATCACTCGTCCTGCCACAGCGC1488760.8088408989806201No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT1271660.6908908202784165No Hit
CGTTGTTGAAAGTTTTAGATTGATTCAATCATTTACTCAGACGCAAAGAT1193090.6482038664155324No Hit
CGTTGTTGAAAGTTTTGACTATTAAATAATCGGTTGACATTAAATAAAAT1181600.64196136800794No Hit
CGTTGTTGAAAGTTTTGATTTTCATTCGAAAATTTTAGTTCAGACTAGAT1105170.6004370726822402No Hit
CGTTGTTGAAAGTTTTAACTTATTTGTATAATAAACTCAGAGATACAGGT1062970.577509880967671No Hit
CGTTGCTGAAAGTTGTATATAAATGCGTTAATTCGCAATAGACATTCTGA1056460.5739730085017504No Hit
CGTTGTTGAAAGTTTTAACTAATTTCGTTATAGGTCTCAGACTGCAACTT1019860.5540882877256074No Hit
CGTTGCTGAAAGTTTTATAAAACTTTTAATATTTCAACAGCTTAACTGCT1013800.5507958995315246No Hit
CGTTGTTGAAAGTTTTGAAGATTTGTGTTCTTCGTGACAAGTTGTAATAA1011480.5495354472856052No Hit
CGTTGTTAAAAGTTTTATTAGTTATAATAAGACTACATTTGTTACAATAA982360.5337145984057887No Hit
CGTTGTTGAAAGTTGTAACTATTATGTTTTTTCAGACGCTGATTGCAACT942350.5119772301474969No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAATA934820.5078861933320772No Hit
CGTTGTTGAAAGTTTTGATTTATTTATGGTTTTACTCAGAAGTTACATAT808890.4394686281042168No Hit
CGTTGTTGAAAGTTTTAAGTTATTTAACTAATTGCTCAGACTGCAATCTT750240.4076041780080204No Hit
CGTTGTTGAAAGTTGTATTAGATGCGTTTTATTACATCGAGTAGACATTC734480.3990417955098779No Hit
CGTTGTTGAAAGTTGTATTTAATTCTTCTGCGTGTGACGGCAGAGTCATA723420.39303291540648605No Hit
CGTTGTTGAAAGTTTTGAAGATTAAAATTCAAATTGACTAGTTTCTAGAG699320.37993942440361594No Hit
CGTTGTTGAAAGTTTTGCTTGTAAATTTTTGGTTTACATAGTAAAAATAC689720.3747237599377424No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACATTA687320.37341984382127397No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT683400.3712901141643756No Hit
CGTTGCTGAAAGTTATATTTGTTAAGATATAACGATACATTCATTTACAA670640.3643576268118186No Hit
CGTTGTTGAAAGTTTTAATTGATTGATTGTATACTCAGACGGCAACCTTC651720.35407842142699275No Hit
CGTTGTTGAAAGTTTTAACTTATTTAGCTAATTGCTCAGACTGCAATCTT620040.33686672868960993No Hit
CGTTGCTGAAAGTTTTATTTATTTTATGCTCAATTAAGAGACTGATTTAC590360.32074163271595074No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATAACTTG565570.30727326582959597No Hit
CGTTGTTGAAAGTTGTATTTCATTAAAGCCCGTAGGCCATTAGACATTCT562600.30565966963546637No Hit
CGTTGTTGAAAGTTTTGACTCGTTTGTATAATACTCGGAGATGCCAACAC552090.2999496036420985No Hit
CGTTGTTGAAAGTTGTATTATGTTTTATAGTTTAAGACATTCTAGACTTT549370.2984718320434343No Hit
CGTTGTTGAAAGTTTTGACGATTGTGTTTTCCACTCAGACCGCAACCTTT536930.2917132001730731No Hit
CGTTGTTGAAAGTTTTGAAGATTTGTGTTCTTCGTGACAAGCTGTAATAA512090.27821766836762524No Hit
CGTTGTTGAAAGTTGTAAATATTAAGTTTTTTCAGACGCTGATTGCAACT507580.2757673926654284No Hit
CGTTGCTGAAAGTTTTGTTTTGTTTATTGCTCTTTCGAGACTTGTTTTAC489630.2660151867110085No Hit
CGTTGTTGAAAGTTTTGAAGTATTATATTTCACTCAGATTTTCTGGTTCA455350.24739091818078493No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAAATACATACATAGACT440900.23954025656288147No Hit
CGTTGCTGAAAGTTTTATTAAACTTTTAATATTTCAACAGCTTAACTGCT405380.22024229803914921No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTGTCTCAGACTACAATCTT401890.2183461866864514No Hit
CGTTGTTGAAAGTTTTGAAGATTTGTATGTAATACTTCTGACAAGTGTAT399770.21719439411690433No Hit
CGTTGTTAAAAGTTGTAATTATTATGTTTTTCAGACGCTGACTGCAACTG392180.21307075939857303No Hit
CGTTGTTGAAAGTTTTGAATAATTTATATTTTCACTCAGACTACAATCTT388450.2110442564342284No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTGTTTTACTCAGAAGTTCCACT383060.20811587815599314No Hit
CGTTGCTGAAAGTTGTATATGTAGATGCGTTGCACGCAATAAACATTCTT367090.19943940299765966No Hit
CGTTGTTGAAAGTTTTGATTGATTTAATCTATTACTCAGACTACAAAGTT356070.19345225482954231No Hit
CGTTGTTGAAAGTTGTATTATGGTTTATAGGCACAAGGCCCATTAAATGA354900.19281659572276397No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTACAATCTT341350.18545490264853615No Hit
CGTTGTTGAAAGTTGTATTAAGTTTAGCGTCCCTTAGGACTTTCATTCAA332260.18051632035741208No Hit
CGTTGTTGAAAGTTTTGACTCATTTGTATACTGCCTTGCGGCACACTCAG280470.15237889716078784No Hit
CATTGTTGAAAGTTGTAACGATTGTTTGTTTCGGAACAGGTAATGCTAGA278860.1515041867659903No Hit
CATTGTTGAAAGTTTTAATTAATTAACTTTGTTTTCAGACAAATACTGCT260210.14137167194426714No Hit
CGTTGTTGAAAGTTTTGACTATTAAGTAATAATCTGGTGTGACAAGTTGA256980.13961681817085342No Hit
CGTTGTTGAAAGTTTTGAAGATTTGTTTTTAAGAAAACTTCTTGACTAAA254650.13835093294111536No Hit
CGTTGTTGAAAGTTTTATTTGATTATAAAATTTAAACATTCTTATACTTA236540.1285117992455976No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTTGAATTTAATCAACAAATTGACAA229610.12474674145929511No Hit
CGTTGTTGAAAGTTTTTTTTAGTTAAATATTAACAAAAAACATACATAAC225680.1226115788185781No Hit
CGTTGTTGAAAGTTTTTATTTGTTATAAAGATTTTACATTCATAGACTAG209930.11405462930425425No Hit
CGTTGTTGAAAGTTTTGAAGTTTAGAAAAGCTGCAAACGCAACTTCTTAT209260.11369061938840683No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTGCAATCTT194590.10572043212649378No Hit
CGTTGTTGAAAGTTTTATTTTGTTATATAATTTAATACATTCATAGACTT185920.10101003515575169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT14473650.0243.467137
GAAAGTT14499900.0243.220698
TTGAAAG12703050.0243.148386
GTTGAAA12728500.0242.213735
TGTTGAA13029800.0242.168154
AAAGTTG5758000.0242.029959
GTTGTTG13608300.0241.831252
TTGTTGA13421650.0241.677643
CGTTGTT13865400.0241.463461
GCTGAAA1827700.0241.058885
TGCTGAA1882100.0237.690644
CTGAAAG1862050.0237.625096
CGTTGCT1932850.0232.044081
GTTGCTG2009250.0228.087372
AAAGTTT9910450.0225.390919
TGACAGT363450.0212.677037
TGAACGT265700.0200.462837
TGGAAGT111850.0195.715167
TGCAAGT292250.0195.538857
GACAGTT414950.0185.86818
Produced by FastQC (version 0.11.2)