FastQCFastQC Report
Wed 10 Aug 2016
000000000-ANER6_l01n02.3320000006707c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANER6_l01n02.3320000006707c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406092
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAATTGACCG13900767.5522604146496715No Hit
CGGAGCTATGGT8947254.861026447113271No Hit
CTTCGGCAGAAT6749843.667177149826264No Hit
ATTCCTGTGAGT6406443.480608485494911No Hit
GACAGGAGATAG5523993.001174828420938No Hit
TTACTGTGCGAT5299772.879356465239878No Hit
CCAGTGTATGCA5110512.7765318134887083No Hit
TATCGTTGACCA5041552.739065957075516No Hit
ATGTGCACGACT4738612.574479145274293No Hit
ATCCCGAATTTG4713572.5608749537924727No Hit
GCACACACGTTA4283502.327218618705155No Hit
CAGGCGTATTGG4208252.286335415470052No Hit
AGCTGGAAGTCC4054742.2029336808704425No Hit
CCTGAACTAGTT3770232.0483598582469327No Hit
AATCCGTACAGC3471151.8858701781996963No Hit
TACACGATCTAC3213611.745949112935No Hit
GGAGACAAGGGA3213431.7458513192262648No Hit
AAGGAGCGCCTT3007491.6339644504656394No Hit
TAGGATTGCTCG2992401.6257660778833443No Hit
ACACGTAAGCCT2865961.5570714304807343No Hit
GAGTGGTAGAGA2823871.5342040015881697No Hit
AATCAGTCTCGT2746491.4921635727997011No Hit
TCACGGGAGTTG2384151.2953048371158853No Hit
GACGGAACCCAT2281891.2397471445866943No Hit
TCGAGGACTGCA2103411.1427792493919948No Hit
CACGGTTGTGAG2100671.1412906118256934No Hit
CAAGCATGCCTA2091871.1365095860653092No Hit
CTTGTGTCGATA1945581.057030465782742No Hit
GTTGGTCAATCT1942981.0556178899899011No Hit
GCGATATATCGC1828860.9936166786518289No Hit
GAGGAATAGCAG1691500.9189892129192878No Hit
TCCTCTGTCGAC1629010.885038497036742No Hit
CGCAGCGGTATA1603000.8709073061245157No Hit
GTCTAATTCCGA1431530.7777479325866675No Hit
ACGCGCAGATAC1198760.651284368240689No Hit
GGAAACCACCAC1182910.642673088888179No Hit
GACTTGGTATTC913480.4962922058631457No Hit
ACATTCAGCGCA883630.4800747491645701No Hit
GGCCACGTAGTA840220.45649016640794793No Hit
ACGTGTACCCAA825210.4483352576962019No Hit
GCTGATGAGCTG769880.41827455822778675No Hit
CTAGCGAACATC707750.3845194297627112No Hit
GAATCTTCGAGC626340.3402895084953395No Hit
CAGCTAGAACGC509490.2768050925747845No Hit
GAACCAAAGGAT451520.2453100853782541No Hit
AAGAGATGTCGA443550.2409799972748153No Hit
ACACCTGGTGAT426050.23147227559223324No Hit
CCACCTACTCCA397670.2160534675149945No Hit
CTATTTGCGACA270260.14683182068197856No Hit
GTATGCGCTGTA204230.11095782852764183No Hit
TCCAAAGTGTTC192690.10468816520095628No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAACT852.439421E-56.00091031
TAACGGG751.7478077E-46.00091031
ATCACGT600.00339090946.00091031
AACGGTT751.7478077E-46.00091031
TCTCGGC550.0091465766.00091031
GTCCGGC600.00339090946.00091031
AGCTGGG1156.80684E-86.00091031
ACGCTCA600.00339090946.00091031
TTACGGG3200.06.00091031
AAGGCGC2300.06.00091031
ACGCGGA550.0091465766.00091031
GCCCACA3750.06.000911
TAGGGTT2100.06.000911
GACGGTT1800.06.000911
AAGGCGT1950.06.000911
TGGAGCG2050.05.99981832
ACCGGCG704.6993853E-45.99981832
CGCGGAC2500.05.99981832
GAGTCAC15650.05.99981832
CGGCATG704.6993853E-45.99981834
Produced by FastQC (version 0.11.2)