FastQCFastQC Report
Wed 10 Aug 2016
000000000-ANER6_l01n01.3310000006707f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANER6_l01n01.3310000006707f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406092
Sequences flagged as poor quality0
Sequence length251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCATTAGAGAAACAAAAAGCTGTCTCGTG13776707.484858817395892No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG13415747.288749833479045No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC9682835.260665870843197No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAATGGGTTGTCTG7073693.8431243307922185No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA4003092.1748723194472785No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCTGGA3920972.130256656328785No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATAAACTTGGTTGGGTTGT3290941.7879623768043755No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTT3229891.7547940105917106No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCCTCGGGGCC2747291.4925982115051908No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGTATTCTATTGCCAGCGCTTA2613281.4197907953518867No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACA2465461.3394804285450708No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGATCAGGACCTGCTCACCC2380291.2932077053618987No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGTCCCTCGGGGCC2212131.2018466494680131No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC2199271.1948598322772699No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCCTAACAAACTCTTTGCGCAAG1901321.0329840794015372No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGATAAA1837160.9981260552212821No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC1727130.9383469342650249No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAACTGC1620950.8806595120789357No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGTTCTTGCTGCCGGCGCTTGA1420160.7715706299848986No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC1357170.7373482649114217No Hit
GTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGCCTCTGGCCCGA1310820.7121663849121258No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTGGACTTCGGTC1259600.6843386417931628No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAAGGGGTTGTAG1250260.6792642349065734No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTCAAATCATCTGTGGCT1188590.6457590236971542No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCATAGG1122320.6097546399311706No Hit
GTTGGTGAACCAGCGGAGGGATCATTAGCGAGTTAATTACAAACTCCAAA1033380.5614336818483793No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAACCTGGGCCTGCGCTACTT1006960.5470797385995897No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGTCCCCTTTGCGG981870.5334483821986764No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGAATTGGGTCGTCCGTGCC885020.48082993391535805No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATTTATGACTGGAGTTGT881860.47911311102867465No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATACAGATGCCTGTCGAGC842760.45787014429787704No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAATATCAGAGACGGGTTGT839820.45627284705520327No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA821000.44604797150856357No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTGCCAGCGGGAGCATCGCGG795050.4319493784992491No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGAATTCGCCCTTCGGGGCTCT733420.3984658992251044No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAAGAGCACCTCTTCATATAT714080.38795850851989655No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAGTTTTGAAGTGGGTTTGAT708100.3847095841963628No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTCAAGGATGCTCTTTTT670980.3645423482616516No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGTTCTGCTGCCGGCGCTTGAC665180.361391217646853No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAACATAGGACGTCCAACTTA571970.3107503754735117No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATGTTAGGACGCTCTTTT547470.2974395651178968No Hit
GTAGGTGAACCCGCGGAAGGATCATTAGAGAAACAAAAAGCTGTCTCGTG521060.28309105485292585No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGGACGTCATACTCCCGTAATA513980.2792445023093441No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGATTCGAGCTTCGGCTCTACC477190.2592565548406473No Hit
GTAGGTGAACCCGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG474380.25772988638761557No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGAGTTGTAGGCTTCTGTCT446870.2427837479025966No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTGAAAGGGGTTGTAG432260.23484615854359522No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAAGTTCAACGGCCAGTGCTG399040.21679778629814522No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGATAGGGTCCCCAACGGGG393940.21402696455064987No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTGCCAGCGCTTA377440.20506254124992965No Hit
GTAGGTGAACCTGCGGAGGGATCATTACCAAGTACAACCCGTCTCCGGAC370050.20104756620797068No Hit
GTAGGTGAACCCGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC361650.1964838598003313No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTCTAAGCTGGAGCT349190.1897143619623329No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATAAATAGGGTGTCCAATT346270.18812793068729636No Hit
GTAGGTGAACCTTCGGAAGGATCATTACCGTAACGCGGCGGTCCAGTAGC323180.17558317105010665No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCACCG323040.175507109276646No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTCACTATGGAGTTGTTG310310.16859092087554492No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATTATAGGACGTCCAATTT298670.1622669277106732No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTCGTGTTTTCAACCTCG288310.15663835647458463No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGTATTCTGCTGCCGGCGCTTA286700.15576364607978707No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGGCCG286020.155394203180121No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAAACAAGGGTGTCCAATTT281110.1527266081251794No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGGGGGTCCCCTTTGCGG273650.14867360219649017No Hit
GTAGGTGAACCCGCGGAAGGATCATTATCGAGTTTTGAATGGGTTGTCTG267980.14559310037133358No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACATAATATTCTTACACACTGTT247380.13440115370497985No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGTATCTTTCGGTCTTGCCAGC247290.13435225685061228No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTAGACTTCGGTC239460.13009823052063416No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGAAAATGACGTGAACTCTTAA232920.12654505910325778No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGTGTTCCCTGATTCCCGGGTG208680.11337550632692697No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTATTGCCAGCGCTTA207090.11251166189976668No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGTGTTGTAGGCTTTGCCTG205520.1116586834402436No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATCAAACAAGTTCATCTTG203830.1107405091748971No Hit
GTAGGTGAACCTGCGGAAGGATCATTATGAATTGTGTAAGCTCTCGTTTA200900.10914864491604194No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACCTTTCAAATCAGGGTGCGGCG199810.10855644967981253No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCTGCCTCCGGGC198790.10800228533031347No Hit
GTAGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC196360.10668207026238923No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAATTCTTTCAGAGACGGGTT191680.10413943383527585No Hit
GTAGGTTAACCTGCGGAAGGATCATTACTGAGATAGGGTCCCCAACGGGG187700.10197710627546576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAA16024900.0244.854773
TAGGTGA16082150.0244.840032
GGTGAAC16770000.0244.520814
ACCTGCG15746500.0244.021369
TGAACCT15754100.0243.57866
AACCTGC15788600.0242.881098
GAACCTG15825250.0242.21727
GTGAACC17154350.0239.443575
ACCAGCG615550.0235.208779
TTGGTGA664300.0232.976822
GTAGGTG17103050.0231.36841
AACCCGC602250.0231.188878
TGGTGAA671100.0229.356663
TGCAGCA1562750.0228.60919245
ACCCGCG617100.0225.962989
GTTGGTG691900.0225.046631
AACCAGC655750.0220.004968
GAACCCG655900.0212.166497
AGGTGGA79600.0205.603013
GAACCAG720800.0201.016947
Produced by FastQC (version 0.11.2)