FastQCFastQC Report
Thu 21 Jul 2016
000000000-ANE69_l01n03.333000000663c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANE69_l01n03.333000000663c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15754003
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGGGCAT527585633.488986894315055No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAGGCG10105166.41434434156195No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG6234493.95740054131004No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC2578191.636530093335643No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCACGGTA1005000.6379331018281512No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGATTCACTGTT770060.4888027506405832No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC760490.4827281040888465No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATTGGGTTCGTATAA659280.41848411479926717No Hit
GGCTACCTTGTTACGACTTAGTCCCCCTCACCAGACTCACCTTCGGCCGG635140.40316102516928554No Hit
GCCCGTCGCTACTACCGACGGGCGGTGTGTACCGAACGGCGATAGTGTAG507130.32190548649762224No Hit
CTACTACCGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGT469860.29824800718903No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTACATAATCAAGTTTGGGCAT413600.26253644867275955No Hit
CAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGT383240.24326515616380165No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGGGCAG360640.22891959586398453No Hit
CCCCCCAATCGGTAGTAGCGACGGGCGACGGGCGGTGTGTACCGAACGGC349860.22207689055283283No Hit
AGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGTC349210.22166429700438678No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAACTAATCAAGTTTGGGCAT346430.2198996661356482No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATAATCAAGTTTGGGCAT332710.21119076846690968No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC317140.20130756608336303No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATGTGGCTCACATTG313750.1991557320383905No Hit
AGTTACCTTGTTACGACTTTTACTTCCTCTAAACGGCCAAGTTTGACCAG280520.1780626803232169No Hit
CTACCGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGT273980.17391135446654415No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCAGCCCTACCGTAGACGGC270060.1714230979897617No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACCCTACCTTGGGCGCC241020.152989687763802No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG227130.14417288101316217No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGATCAAGTTTGACCAG214740.13630821322047482No Hit
GGCTACCTTGTTACGACTTAGTCCCCCTCACCAGACTCACCTTCGACCGG194170.12325121431042002No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGGGCAT157930.10024753708628847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG12995700.092.90839
CTTGTTA12755850.092.898817
ACCTTGT12746300.092.897325
CCTTGTT12783150.092.891966
AAACCTT11400050.092.850023
AACCTTG11462000.092.7997744
GAAACCT11423050.092.759472
TTGTTAC12768450.092.729628
GGAAACC11418950.092.695121
GATACCG224500.092.659622
GGCTACC618000.092.5602651
ACCGTGT248050.092.17945
CCGTGTT249600.092.053756
CGTGTTA249650.091.9236457
GTTACCT567950.090.9652252
GGATACC266950.090.4396741
GGTTACC532100.089.741411
GTGTTAC255850.089.641578
TTACCTT576900.089.5459443
TACCTTG1342700.089.2086264
Produced by FastQC (version 0.11.2)