FastQCFastQC Report
Thu 21 Jul 2016
000000000-ANE69_l01n02.332000000663cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANE69_l01n02.332000000663cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15754003
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCTCCGCT13993768.88266937615792No Hit
AATCCGTACAGC7362884.673656593819361No Hit
GCGGATGTGACT3978552.5254216341078517No Hit
AGTTCAGACGCT3524132.236974310592679No Hit
ACACCTGGTGAT3426882.1752439681520945No Hit
AATCAGTCTCGT3410612.164916434254837No Hit
GCTGATGAGCTG3355032.1296365120661713No Hit
TAGCTCGTAACT3247002.0610634643144348No Hit
TTCAGACTACCA3225562.0474542248087677No Hit
TTAGGGCTCGTA3198932.030550584508585No Hit
CACGCCATAATG3119601.9801951288190056No Hit
CTGCTAACGCAA3118191.9793001181985304No Hit
GCACACACGTTA3050131.9360983998797006No Hit
TTACTGTGCGAT3035331.9267039621612363No Hit
CCTCGTTCGACT2998871.9035606378899381No Hit
ACGCGCAGATAC2977591.8900529598731193No Hit
CCAGTGTATGCA2802251.7787542632815294No Hit
AGCTGTTGTTTG2716551.7243553908171783No Hit
TATCGTTGACCA2694661.7104605096241252No Hit
TCACGGGAGTTG2690051.7075342692266848No Hit
GTTGGTCAATCT2645991.6795667742350946No Hit
GACTTGGTATTC2632621.6710800423232115No Hit
TCCGACACAATT2625631.666643074779153No Hit
ATTTCGACATGC2572421.6328675321440524No Hit
GTATGCGCTGTA2505981.5906941238998114No Hit
AGGCTACACGAC2495691.5841624506482572No Hit
ACCGTGCATAGG2453161.5571661373937786No Hit
TACACGATCTAC2394531.5199501993239433No Hit
GGATCGCAGATC2355991.495486575697618No Hit
TAATGACCACGC2308571.465386289440214No Hit
CAGGCGTATTGG2233681.417849165066174No Hit
GACTTTCCCTCG2229271.4150498765297936No Hit
TGAGTCACTGGT2096721.330912530612061No Hit
CAGTGCATATGC2010491.2761772357159002No Hit
AACGGATAAACG1976111.2543542107996297No Hit
GGATGGTGTTGC1713211.0874759894358277No Hit
GAGTGAGTACAA1602021.016897102279338No Hit
TAGGATTGCTCG1594821.0123268352811663No Hit
ATGTGCACGACT1393200.8843466641462491No Hit
GGAGACAAGGGA1350850.8574646075667245No Hit
CTTATCAAGGCG1335990.848032084289942No Hit
ATCGAATGCGGC1098580.697333877618279No Hit
TGCCTACAAGCA1021200.6482162025740378No Hit
CAGGGTGATACC972950.6175890660932336No Hit
TGCCCATTTAAG808050.5129172566489927No Hit
TGAGAACCTCCA753840.47850695470859056No Hit
ATCCCGAATTTG562780.35722984183765866No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG550.0091607156.00000054
AGTCCCG2600.06.00000055
CACGGAG650.00126197216.00000052
TTAGCGT550.0091607156.00000051
TTATGGC2200.06.00000051
AGGACTC1101.8153514E-76.00000053
AAGACGC650.00126197216.00000055
GCTTCCG650.00126197216.00000054
CTACGGG550.0091607156.00000051
TGCGACT6300.06.00000056
CCGAGCA650.00126197216.00000052
AGTCTGG650.00126197216.00000055
GTATCGG650.00126197216.00000056
CTACAGG550.0091607156.00000054
CGACTTG550.0091607156.00000055
CTGGGGC550.0091607156.00000055
AGAGGCT550.0091607156.00000056
TGTACGC1303.6470738E-96.00000055
TAATGGC550.0091607156.00000051
ACAAGCT650.00126197216.00000056
Produced by FastQC (version 0.11.2)