FastQCFastQC Report
Thu 21 Jul 2016
000000000-ANE69_l01n01.331000000663cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANE69_l01n01.331000000663cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15754003
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCGG681145043.23631270096877No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACCGCTGTTTCGAC13785088.750207804327573No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAAGA5172023.2829878222062034No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG3703392.350761263661052No Hit
GCTTCAACCGATTGGATGATCCGGCAAACTGTACGGACATTTGGAATCAC1194720.758359637229979No Hit
GCTCCTACCGATTGAATGATCAGATGAAATGTTCGGATGGAATAGTTGCA1067520.6776182535956099No Hit
GCTCCTACCGATTTCGAGTGATTCGGTGAGTCTTTTGGACCTGTCACAAG825370.5239112878168171No Hit
GGTAGTAGCGACGGGCGTAGGTGAACCTGCAGAAGGATCATGCTGACTGA752810.47785315262412986No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGTCCGGCGCCAATTGTCCGGCCG689190.43746976562083933No Hit
GCCCGTCGCTACTACCGATTGGGGGGGTAGGTGAACCTGCAGAAGGATCA569130.3612605634263241No Hit
ACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGGCTAACCTCGTGAGG478440.3036942420285181No Hit
ACACCACGAAAGTTGGTTTTACCCGACACCGGTGAGCTAACCCGCAAGGG472280.29978412470786No Hit
GCTACTACCGATGAATGGTTAAGTGAGGCCTTCGGATTGGCCGACGCGTG356690.22641229660804305No Hit
GCTTCAACCGATTGGATGATCCGGCAAATTTTGCAGACATTTGTAATTTT308200.19563281789396636No Hit
ACACCACGAAAGTTGGTTTTACCCGACACCGGTGGGCTAACCCGCAAGGG279900.17766912955392988No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGCCCGGCGCCAATTGTCCGGTCG260380.1652786279144418No Hit
GCTACTACCGATTGAATGATTTAGTGAGGCCTTCGGACCGGCGGGCGCGG256650.16291097570566668No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTTGGGGG241500.15329437223034678No Hit
ACACCACGAAAGTTGGTTTTACCAGACGTCGCCGGACTAACCCTTCGGGG233360.14812743148519142No Hit
ACACCATGGGAGTTGATTTTACCTTAAGTCGGTGAGCTAACCAGCAATGG215180.13658750731480754No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGAGG194720.12360033192833593No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAAGA188900.11990603277148038No Hit
TCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCGG168930.10722988944460655No Hit
GCTTCAACCGATTGGATGATCCGGCAAACTGTACGGACATTTGGACTCAC165530.10507170780658097No Hit
GCTACTACCGATTGCATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCGG159240.10107907177623363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA8495500.0107.210919
CATGGGA596400.0107.172745
CGGGACT1523700.0107.160587
TGGGAGT593900.0107.073037
GGGACTG1528400.0107.066168
GGGAGTG522500.0106.739198
AACCGAT204000.0106.710596
ATGGGAG599700.0106.672436
ACCCAAG128450.0106.469376
GAAAGTT259450.0106.291128
GGACTGA1538300.0106.241149
CGATCGA11650.0105.88169
GTACCGA681900.0105.673955
CCGGGAC1542800.0105.295136
CAACCGA206350.0105.0794755
AAAGTTG262800.0104.9566049
CCGATTT103350.0104.5123448
GAAAGTC24950.0104.4682548
CGCTTGA34850.0104.030949
ACCGATT9114850.0103.8958747
Produced by FastQC (version 0.11.2)