FastQCFastQC Report
Thu 21 Apr 2016
000000000-ANABT_l01n03.3330000005294b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANABT_l01n03.3330000005294b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15664504
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA317938820.296767775092018No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA10654006.801364409623184No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA5007773.1968902430616377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2917581.8625422164659666No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG2341501.4947808114447798No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA2183961.3942094815131076No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1962071.2525580126890707No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1906271.2169360740691182No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAT1439190.9187587426962258No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1389390.8869671200569134No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1301490.8308529909405368No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA1257100.802515036543768No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1248310.7969036236321303No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG964390.6156530714282431No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG895340.5715725183510438No Hit
CCTGTTTGATCCCCACGCTCTCGCACATCAGCGTCAGTTACAGACCAGAA823110.5254618978041053No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG768010.4902868293818943No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTGTTATCCCAGGA739420.4720353737341444No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT700280.44704894582043586No Hit
ACTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA494330.3155733497849661No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG470550.300392530781696No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA469920.2999903476037288No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTT465480.29715591377805517No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA386250.2465765912537033No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG375940.23999483162697013No Hit
CCTGTTCGCTACCCATGCTCTCGCTCCTCAGCGTCAGTAACTGCCCAGTA373140.23820735083600478No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCCGAA369320.2357687163283306No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA361240.23061055747440198No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA340070.21709592592271035No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA333900.21315708432261882No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG306360.19557593397148101No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA305440.1949886188544495No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG294210.1878195441106849No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA281580.1797567289714376No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA267120.17052566745809505No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT249020.15897088091649758No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG248920.15890704231682024No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA240630.15361482240356925No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA236830.15118895561583054No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGT236790.1511634201759596No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGTA223430.14263458325906778No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA207070.13219058835185588No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAC205920.13145644445556656No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGCCCAGAA201820.12883906186879585No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCCGCGTCAGTTACAGACCAGAA190560.12165083554512802No Hit
CCTGTTTGATCCCCACGCTTACGCACATCAGCGTCAGTTACAGACCAGAA190020.12130610710687041No Hit
TCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA187600.11976121299467893No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGACCAGTA186510.11906537225819598No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG182990.11681825354955382No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG181550.1158989777142002No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGCA176160.1124580771915919No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCCGTTACAGACCAGAA164400.10495065786953739No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTCCAGACCAGAA164130.1047782936504086No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG157980.10085221977025254No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATAATGGCCAGAA157950.10083306819034933No Hit
CCTGTTTGATCCACACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA157690.10066708783118827No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG157270.10039896571254346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA4751550.0244.120853
TTTGATC4555700.0243.768465
TGTTTGC4308300.0243.518593
GATCCCC4600600.0242.747348
CTGTTTG9066350.0242.52742
CTGTTCG3299500.0242.512482
TGATCCC4587750.0242.220357
GTTTGAT4802050.0241.979614
TTTGCTC3786650.0241.971985
GTTTGCT4382350.0240.798664
ATCCCCA4590450.0240.67699
TGTTCGC3233150.0240.513983
CCTGTTC3249200.0239.213551
TTGATCC4746900.0237.68656
CCTGTTT9073050.0236.928181
GTTCGCT3538400.0235.811814
TTGCTCC3954400.0234.811286
CTCCCCA4864050.0230.878899
TGCTCCC3977150.0230.683727
GCTCCCC5318400.0229.922428
Produced by FastQC (version 0.11.2)