FastQCFastQC Report
Thu 21 Apr 2016
000000000-ANABT_l01n02.3320000005294e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANABT_l01n02.3320000005294e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15664504
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCAAAGGAT4021462.5672437505841232No Hit
GCACACACGTTA3850992.4584180897141716No Hit
CTTCGGCAGAAT3806382.4299396903981125No Hit
ATCCCGAATTTG3683312.3513735257752177No Hit
GTCAATTGACCG3624262.313676832665752No Hit
GAAGTTGGAAGT3590582.2921759922944256No Hit
AATCAGTCTCGT3527012.251593794479544No Hit
GGAGACAAGGGA3462332.2103029882082446No Hit
CACGGTTGTGAG3433832.1921089873002044No Hit
ACACCTGGTGAT3369092.1507798778690983No Hit
CCTCGTTCGACT3312892.1149025848504364No Hit
CCAGTGTATGCA3287862.0989237833511996No Hit
GGCCACGTAGTA3201962.0440864262283696No Hit
AGGCTACACGAC3177062.028190614908713No Hit
TCTAGCGTAGTG3151432.011828781811413No Hit
TTACTGTGCGAT3110591.9857570977031895No Hit
AGCTGGAAGTCC3018061.926687241421752No Hit
TCGAGGACTGCA3005341.918566971542795No Hit
AATCCGTACAGC2819091.7996675796437605No Hit
ACACGTAAGCCT2785161.7780071427732407No Hit
TCCAAAGTGTTC2589431.6530558516247944No Hit
AAGAGATGTCGA2566951.63870493441733No Hit
ACGCGCAGATAC2558021.6330041474661439No Hit
CCTGAACTAGTT2500061.596003295093161No Hit
ATTCCTGTGAGT2493631.5918984731339083No Hit
GAGGCTCATCAT2456031.5678951596552309No Hit
GAGTGGTAGAGA2451571.5650479581096215No Hit
TATCGTTGACCA2305481.4717861478410041No Hit
GCGATATATCGC2206051.4083114281818307No Hit
TACACGATCTAC2176351.3893513640776625No Hit
TAGGAACTGGCC2158541.3779817094751292No Hit
GAGGAATAGCAG2128731.358951422911316No Hit
GAACTAGTCACC2092801.3360142140472497No Hit
TACAGATGGCTC2092081.3355545761295728No Hit
TAGGATTGCTCG2019991.2895333296221827No Hit
GTTGGTCAATCT1731621.1054419597326541No Hit
TCCCAGAACAAC1704891.0883779020389026No Hit
ACGTGTACCCAA1693911.0813684237943315No Hit
GACTTTCCCTCG1613641.0301251798333353No Hit
CTAACCTCCGCT1538610.9822270784954314No Hit
ATGAGACTCCAC1501220.9583578260760762No Hit
GACAGGAGATAG1436610.9171117068245506No Hit
CACGCCATAATG1373530.8768423181480881No Hit
CAAGCATGCCTA1367500.8729928505875448No Hit
CAGGCGTATTGG1292500.8251139008295444No Hit
AGGCATCTTACG1240270.7917710002180726No Hit
CTATTTGCGACA1170200.7470392934241646No Hit
GACGGAACCCAT1030790.658041901613993No Hit
GACTTGGTATTC928640.5928307720435961No Hit
CAGCTAGAACGC862780.5507866702961038No Hit
CAGCGGTGACAT657910.42000053113714936No Hit
TGAGTCACTGGT584600.3732004537136956No Hit
ATGTGCACGACT499540.31889934082815513No Hit
AAGGAGCGCCTT295240.18847708168736144No Hit
TAGCTCGTAACT219440.14008742313194214No Hit
GAATCTTCGAGC166280.10615082354347129No Hit
CTAGCGAACATC159620.10189917280496082No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGTC2400.06.05
GTACCGC1900.06.06
CGAACAG600.00339656726.05
GCGACTC1405.18412E-106.03
GGTGTCG1054.8308175E-76.05
CGCGGAC3100.06.02
CGGCCTG806.539384E-56.04
CGGCCGA1653.6379788E-126.04
GGCGTGT550.009160716.03
CCAGCTC550.009160716.03
TCGGCCG2250.06.03
AACGGCT953.4298464E-66.06
AGGCCTG953.4298464E-66.04
AGGCCCA650.00126197086.02
CTACGCG953.4298464E-66.04
TTGACGG1054.8308175E-76.06
TCATGCC953.4298464E-66.04
CGTACCG1800.06.05
TCGAGAC550.009160716.01
GGCGACG953.4298464E-66.01
Produced by FastQC (version 0.11.2)