FastQCFastQC Report
Thu 21 Apr 2016
000000000-ANABT_l01n01.33100000052941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ANABT_l01n01.33100000052941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15664504
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA502388232.0717591824165No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA173040011.046631288165907No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTATTGGGCGTAAAGCGAGTGTA7371954.706149648913238No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA6437764.109775834587549No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA4781993.052755452710153No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2642541.6869605319134267No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2350361.5004369113761917No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2014171.2858179231209619No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA1825591.1654310918494453No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1574771.0053111161387553No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1441020.919926989070321No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA1371590.875603849314348No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA834190.532535214648354No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA750310.4789873972390061No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA736360.47008191258401794No Hit
TACGTAGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA708600.45236031731359No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA692580.44213337364528105No Hit
TACGTAAGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA690870.4410417335907987No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCTCGTA600410.38329333632268214No Hit
TACGTAGGTGGCAAGCGTTATCCGGCATTATTGGGCGTAAAGCGCGCGTA525180.33526755778542366No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA523920.33446319142948927No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA523800.33438658510987646No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA483170.30844896206097555No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA425450.2716013223272183No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA399600.2550990443106274No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA383690.24494232310196354No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA365300.23320240462130176No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA354490.22630145199618196No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA354430.22626314883637555No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA353280.2255290049400862No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA348470.22245836829560642No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA334240.21337413556152177No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA329470.21032903435691291No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTCACTGGGCGTAAAGCGCGCGCA328830.20992046731897798No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA319260.2038111133298571No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA311750.19901683449408933No Hit
TACGTAGGTGGCAAGCGTTATCCGGACTTATTGGGCGTAAAGCGCGCGTA273030.1742985286990255No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA269350.17194926823089962No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCGCGTA264330.16874457052709746No Hit
TACGGGAGGTGCAAGCGTTATCCGGTTTTATTGGGTTTAAAGGGTGCGTA259060.16538027632410193No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA249050.15899003249640078No Hit
TACGTAAGGACCGAGCGTTGTCCGGGATCATTGGGCGTAAAGGGTACGTA243060.15516610037572845No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA241070.1538957122421495No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA211830.135229305696497No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA207170.13225442695153322No Hit
GACGAGTGAGGCTAGCGTTATTCATAATTATTAGGTCTAAAGGGTACGTA206480.13181394061375962No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA202820.1294774478655692No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA196410.12538539362625206No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTACAGCGCGCGTA185190.11822270274245517No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA184340.11768007464519784No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA183360.11705445636835995No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA183200.11695231460887621No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA180970.11552871383607166No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA179360.11450091238126658No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA173970.11106001185865828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCC908950.0244.649585
GGTCCCG912150.0244.409077
AGGTCCC914000.0244.276266
GTAGGGT3786350.0243.628224
GTAGGTC997750.0243.29344
TACGTAT427450.0242.993911
CGTAGGG4555150.0242.687233
ACGTATG427850.0242.509052
GTGCGAG3733100.0242.410939
TAGGGTG3788700.0242.374575
TAGGGCG390600.0241.926515
TCAAACA4631400.0241.90271245
TCCCGAG929950.0241.28539
GTCCCGA929100.0241.097298
GTGGCAA6038600.0241.076648
AGGTGGC6124150.0240.69546
ACGTAGG11829350.0239.798372
TCACAAG75600.0239.32879
TGGCAAG6103700.0239.045299
GGTGGCA6096000.0238.951347
Produced by FastQC (version 0.11.2)