FastQCFastQC Report
Wed 18 May 2016
000000000-AN4CJ_l01n02_grads_set03_rna_digest.35200000055b27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN4CJ_l01n02_grads_set03_rna_digest.35200000055b27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869416
Sequences flagged as poor quality0
Sequence length26
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGCTATATGACCCATGCATCA49050.12676331518761488No Hit
TTGAGTCATGATGGCATATGGAGTTT47880.12373960308222223No Hit
TTGAGTTATGGTCTAGAAAAACTCCA45550.1177180225646454No Hit
TTGAGTTAGCTATCTCCCTAATGAGT42260.10921544749905411No Hit
TTGAGTAGCTGGTTCCCTCCGAAGTT40940.1058040799955342No Hit
TTGAGTCTATATTATTCAAGCCTTTT40600.10492539442644574No Hit
TTGAGATTATGGTTAGTCATCAAGGT40230.10396917777773183No Hit
TCAAGAAGTAAAAGACACTTGAAACC39930.10319386698147731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA762300.019.7664071
TTGAGTT858100.019.7435321
TTGAGTG335550.019.7197741
TTGAGTC380550.019.624141
TTGAGCG8350.019.161591
TTGAGCT18500.018.4323481
CGCCTTA18850.018.2494893
GCCTTAC18800.018.2448354
GCCGCCT18300.017.5955491
TTGAGAG24700.017.5302851
TCAAGCA11800.017.4575481
TTGAGCC11300.017.3450531
TTGAGAT32000.017.2811721
TTAAGTC8250.017.2120441
ACTTGAG16500.017.15143815
CCGCCTT20500.016.8779722
CACTGAC19150.016.81454710
CCTTACA20400.016.8138685
TTACACT20600.016.699177
CTTACAC20450.016.6260576
Produced by FastQC (version 0.11.2)