FastQCFastQC Report
Wed 18 May 2016
000000000-AN4CJ_l01n02_grads_set03_dna_digest.35200000055b1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN4CJ_l01n02_grads_set03_dna_digest.35200000055b1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104532
Sequences flagged as poor quality0
Sequence length26
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTCCTCCTATTTTTCGAAT57500.18521310136278188No Hit
TTGAGTCCGTTAGAAGATTCTATTCA40930.13183951719615067No Hit
TTGAGTGGAGTAGGGCTGAGACTGGG33940.10932404626526639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA580150.019.7569581
TTGAGTG258850.019.702531
TTGAGTT609850.019.6310581
TTGAGTC280300.019.628971
TTGAGCC17400.018.9080471
CGCCTTA23200.018.8362063
TTGAGCT23300.018.7982851
CTTACAC23700.018.5232076
TTGAGCG10000.018.51
GCCTTAC24200.018.2644624
CCTTACA24000.018.255
TTGAGAT24350.018.1108821
TTGAGAG28700.018.048781
TTACACT25100.018.0079677
TCTCGAG502.799292E-618.0000028
TTAAGCC3500.018.01
TCAAGGA10850.017.972351
TTGAGGT20850.017.8896881
GCCGCCT24000.017.8751
TCAAGTG17700.017.8531071
Produced by FastQC (version 0.11.2)