FastQCFastQC Report
Wed 18 May 2016
000000000-AN4CJ_l01n01_grads_set03_rna_digest.35100000055b2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN4CJ_l01n01_grads_set03_rna_digest.35100000055b2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869416
Sequences flagged as poor quality0
Sequence length26
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTGCTATATGACCCATGCATCA51580.13330176956936138No Hit
TTGAGTTATGGTCTAGAAAAACTCCA49560.12808134354124756No Hit
TTGAGTAGCTGGTTCCCTCCGAAGTT47890.12376544677543072No Hit
TTGAGTCATGATGGCATATGGAGTTT46740.12079342205645503No Hit
TTGAGTACACATTTAATTTAGACTCA39680.10254777465126519No Hit
TTGAGTTAGCTATCTCCCTAATGAGT38940.10063534135383737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTT837500.019.7767161
TTGAGTA728650.019.7653181
TTGAGTG316900.019.6402651
TTGAGTC355850.019.5869051
TTGAGCG8600.019.4186041
TTAAGTA17400.018.5057471
TTGAGCT17300.017.976881
GCCTTAC14000.017.6428574
TTGAGCC11150.017.3991031
TTGAGAG22650.017.2626931
CGCCTTA12050.017.261413
ACTTGAG15350.017.19869815
CCTTACA16700.017.1856295
TCAAGCA10800.017.0370371
TTGAGAT29050.016.867471
GTACTAA19000.016.8421065
TTGAGGG8100.016.7901231
TTAAGTC10100.016.7326721
GAGTATC128200.016.708273
GTATCGG14550.016.7010335
Produced by FastQC (version 0.11.2)