FastQCFastQC Report
Wed 18 May 2016
000000000-AN4CJ_l01n01_grads_set03_dna_digest.35100000055b1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN4CJ_l01n01_grads_set03_dna_digest.35100000055b1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104532
Sequences flagged as poor quality0
Sequence length26
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTCCTCCTATTTTTCGAAT52470.16901098136530723No Hit
TTGAGTCCGTTAGAAGATTCTATTCA37370.1203724103987332No Hit
TTGAGTGGAGTAGGGCTGAGACTGGG34530.11122449374011928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA549800.019.7471791
TTGAGTT587050.019.6814561
TTGAGTG243200.019.6463811
TTGAGTC255750.019.5816231
TTGAGCT22750.018.9890121
TTGAGCG13900.018.7050341
TTGAGCC17500.018.2857151
CTTACAC21400.018.1308426
TTAAGTA11200.018.0357151
GCCTTAC18150.017.9063364
CCTTACA21800.017.7981665
CACTGAC24050.017.79625910
CGCCTTA15200.017.6973673
TTGAGAT21550.017.5406021
TTGAGAG23950.017.369521
TTGAGGT18150.017.3553711
TTACACT24300.017.3251047
ACTGACT24350.017.24845911
TCAAGTG12700.017.2440951
TTGAGGG14150.017.2438161
Produced by FastQC (version 0.11.2)