FastQCFastQC Report
Fri 20 May 2016
000000000-AN12D_l01n03.3330000005602d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN12D_l01n03.3330000005602d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15635020
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTTTAAGTTATTTAACTAATTGCTCAGACTGCAATCTT5146823.291853799995139No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT4200672.6867058692601606No Hit
CGTTGTTGAAAGTTTTAACTTATTTAGCTAATTGCTCAGACTGCAATCTT3077871.9685743926135049No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT2977451.9043467804966032No Hit
CGTTGTTGAAAGTTTTAAATAATTTAAATAATCGCTCAGACTGCAATCTT2353481.505261905645148No Hit
CGTTGCTGAAAGTTTTATTAAACTTTTAATATTTCAACAGCTTAACTGCT2056021.3150095107009776No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTTGAATTTAATCAACAAATTGACAA2015541.2891189138229435No Hit
CGTTGTTGAAAGTTGTATAGTTTACGATTGACGCAACTGAGCGCAAGACA1927291.2326751101053917No Hit
CGTTGCTGAAAGTTTTATTTTATTTATGCTCAATTAAGAGACTGTTATAC1538980.9843159778497245No Hit
CGTTGTTGAAAGTTTTTATTTGTTATAAAAATTTTACATTCATAGACTAG1507070.9639066659332703No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAATACATTCATAGACTT1470930.9407918889774365No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAATA1462070.9351251229611476No Hit
CGTTGCTGAAAGTTGTATTTAAAATGCGTTATACGCGATGTTTACATTCT1438610.9201203452250142No Hit
CGTTGTTGAAAGTTTTGACTATTAATAAATATAATCAGTTGACTAGTTTA1391760.890155561041815No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATAACTTG1381820.8837980379941952No Hit
CGTTGTTGAAAGTTGTATTATGGTTTATAGGCACAAGGCCCATTAAATGA1345830.860779199514935No Hit
CGTTGCTGAAAGTTTTATAAAACTTTTAATATTTCAACAGCTTAACTGCT1278330.8176068850567508No Hit
CGTTGTTGAAAGTTTTGACTATTAAATAATCGGTTGACATTAAATAAAAT1273280.8143769563454348No Hit
CATTGTTGAAAGTTTTGACTGATTTTATATTCAGACTCAGACTGCATCAC1255640.8030945915003627No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTACAATCTT1236710.7909871557567564No Hit
CGTTGTTGAAAGTTTTGAAGATTTTAATTTTTGTTAAAAATTTTCATGAC1152460.7371017114145041No Hit
CGTTGCTGAAAGTTGTATATAAATGTGTTATAACACAAGTATGACATTCT1134440.7255763024287785No Hit
CGTTGCTGAAAGTTGTATATGTAGATGCGTTGCACGCAATAAACATTCTT1107970.708646359262732No Hit
CGTTGTTGAAAGTTGTATTAGATGCGTTTATAGCAAGTTACATTCAGAAC1105890.707316012387576No Hit
CGTTGTTGAAAGTTTTGATTTATTTATGGTTTTACTCAGAAGTTACATAT1029620.658534494999047No Hit
CGTTGTTGAAAGTTTTGTTTTGTTATAATTTTGTATACATTCATAGACTT1000680.6400247649187529No Hit
CGTTGCTGAAAGTTATATTGTGTTTGAATATAACAAGACATTCTATGACA981170.6275463670657281No Hit
CGTTGTTGAAAGTTGTATTAGATGCGTTTTATTACATCGAGTAGACATTC843170.5392829686178847No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACTAAA842670.5389631736959722No Hit
CGTTGTTGAAAGTTTTAAATAATTTAATAATCGCTCAGACTGCAATCTTC833910.5333603666640657No Hit
CGTTGTTGAAAGTTTTAAATTATTTTAAAATTTCCGTTAGGAATTTTGGT833530.5331173225234122No Hit
CGTTGTTGAAAGTTTTGTTTTTTTGATTTTAATCAAGTATAATTCAGACG811710.5191614721311517No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTGAATTTAATCAACAAATTGACTTT809630.5178311252559958No Hit
CGTTGTTGAAAGTTGTAATTATTAATTTGTTACTGACGCTGATTGCAATT789870.5051928299420149No Hit
CGTTGTTGAAAGTTTTGTTTTTGATTGGTATATCAAAAGTCTGATTCAGA767960.4911794164638101No Hit
CGTTGTTGAAAGTTGTAATTATTAGGTTTTTCAGACGCTGATTGAAAATT731520.46787276255482885No Hit
CGTTGCTGAAAGTTGTATTTATTTGCTTATTATGCAAGGTTACATTCTAA714120.4567438992722747No Hit
CGTTGTTGAAAGTTGTATTTGTGCGAGTTAACGCAAGGTACATTCTGATA660670.42255782211983106No Hit
CGTTGTTAAAAGTTGTAATTTAATGCTGTTAAGCTTTAAACATTCAATAA629940.4029032262190902No Hit
CGTTGTTGAAAGTTTTGTTTGTTATAAATTTGTTACATTCATAGACTTTG593480.3795837805132325No Hit
CGTTGTCGAAAGTTGTATTAACTTTTTAGGGTCTGTCAAGACCATGATTA581070.37164647055136485No Hit
CGTTGTTGAAAGTTGTTTCGTTTATGGTTTATTACATTCTGGACTGTTGT556030.355631140861988No Hit
CGTTGCTGAAAGTTTTGATTTATTTGCTTATGCCACTCAGAAATCCACTG539040.3447645094154021No Hit
CGTTGTTGAAAGTTGTATTAGTTTTAAAGGCTATACAAGCCCAGTAAGAC530340.339200077774125No Hit
CGTTGCTGAAAGTTTTAATATTTGCTTGCGCTGCTCAGAAGATCCTCTGT517460.33096216058565964No Hit
CGTTGTTGAAAGTTTTAACTAATTTCGTTATAGGTCTCAGACTGCAACTT513440.3283910094134833No Hit
CGTTGTTGAAAGTTTTGTTTTGTTATAAAATTTAAACATTCTTTGACTTA477640.30549369300455004No Hit
CGTTGCTGAAAGTTGTATATAATGCGTTTAACGCAATGACATTCTATAAA471150.3013427549181261No Hit
CGTTGTTGAAAGTTATATTTGTGCGAGTTAACGCAAGGTACATTCTGATA467410.29895068890222076No Hit
CGTTGTTGAAAGTTTTGATTGATTTAATCTATTACTCAGACTACAAAGTT462030.29550969554244255No Hit
CGTTGTTGAAAGTTTTGCTTTTAATTAAAAAATAGTACTCAGAGTAGACA450570.28817999593220855No Hit
CGTTGTTGAAAGTTTTAATGATTTAATCATAGACTCAGACTCACTATTCA438830.28067121116570365No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT433720.27740290706375814No Hit
CGTTGTTGAAAGTTTTGATTGAATTCGCTTCAATATCCGACGACAACTGA421110.2693376791331255No Hit
CGTTGCTGAAAGTTTTGTTTTGTTTATTGCTCTTTCGAGACTTGTTTTAC419590.26836550257051156No Hit
CGTTGTTGAAAGTTGTTATTGTTTATGGTATTTGACATTCTGGACTTTTG416400.26632521096871No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTTTTATACTCAGAAGTTCCACT400320.25604060628000475No Hit
CGTTGCTGAAAGTTGTATATAGTTTATAAGGCAACTAAGCCTAATAATGA388630.24856380100569106No Hit
CGTTGTTGAAAGTTGTATTTCAAATATCGTCCCGTAGGACTCAAAAACAT377220.24126608088764837No Hit
CGTTGTTGAAAGTTGTATTGTTTAATAACAAAAACGTTCTACTATGACAT367250.2348893701447136No Hit
CGTTGCTGAAAGTTATATTGTGTTTAAATATAACAAAACATTCTATGACA359630.23001569553476744No Hit
CGTTGTTGAAAGTTTTGATTCATTTGTATACTGCCTTGCGGCACACTCAG347730.22240457639325054No Hit
CGTTGCTGAAAGTTGTATATAGACGCGTTGAACGCAAACAGACATTCTTT341980.21872693479125707No Hit
CGTTGTTGAAAGTTTTGATTTATTCAAAATTTTAACTCAGACGACCGGTT340090.21751810998642793No Hit
CGTTGTTGAAAGTTGTTTCGATTTGTGGGCGGATCAGAGTCCGCTTGGCA330940.2116658629154296No Hit
CATTGTTAAAAATTGTAATTTAATAAGTCTGCTTTTTGACGGCAAGACTA322560.20630610002417651No Hit
CGTTGTTGATAGTTGTAATTAAAAAATAGATTAGTTAGATATAAGTAATT318230.20353667600041445No Hit
CGTTGTTGAAAGTTGTAACTATTAATTTGTTACTGACGCTGATTGCAATT314040.2008567945547879No Hit
CGTTGTTGAAAGTTGTATATAGTTTTAGAGGACATAAGTCCAGTAAACAA311690.19935375842179925No Hit
CGTTGTTGGAAGTTGTATTAGTTTTAAAGGCTATACAAGCCCAGTAAGAC307200.19648200002302524No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACATTA306470.19601509943703305No Hit
CGTTGTTGAAAGTTGTAATTATTAATTTATTCAGACGCTGATTTAAGAAA300650.19229268654597179No Hit
CGTTGTTAAAAGTTTTGATTTATTTTTGCGTTTGCCACTCAGAGAATACT276250.17668669435664297No Hit
CGTTGTTGAAAGTTTTGACTATTAGTAATAATCTGGTGTGACAAGTTGAT265750.16997100099648096No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTTCAATCTT255190.16321693224568948No Hit
CATTGTTAAAAGTTTTGATTATTTTTGTTTTGACTATAAAATTATTGTTT243330.15563139669792556No Hit
CGTTGTTGAAAGTTTTAACTGATTTAGTCAAGTACTCAGACTGCAATCTT225550.1442594892747179No Hit
CGTTGTTGAAAGTTGTATATATTTAAAATGCGTTGTACGCAAAGTAAGAC225220.14404842462625567No Hit
CGTTGTTGAAAGTTTTAATAATTTATGCTTATCGCTCAGACTGCAAGGTA224660.14369025431371368No Hit
CATTGTTAAAAGTTGTCAATTATTAAGCAGAGAGAGCAAAGCAAAGCAAA222850.14253259669639054No Hit
CGTTGTTGAAAGTTTTGTTTTTGTTAAGATAACAAGACGTTCGTTACACT218440.1397120054851225No Hit
CGTTGTTGAAAGTTGTAACTATTAAGTTTTTTCAGACGCTGATTGCAACT215270.13768450568019738No Hit
CGTTGTTGAAAGTTTTGACTATTGTAATAATAAATCAAGTTTGACTGTAA213150.13632857521128852No Hit
CGTTGCTGAAAGTTGTATTTATATGCGTTATGCAATAAAGACATTCTAAG209240.13382777892193293No Hit
CGTTGTTGAAAGTTTTTATTTGTTATAAAAATTTTACATTCATAGACCAG208950.1336422978672237No Hit
CGTTGTTGAAAGTTTTATTTGTTATAATAAAAGACGTTCATGACTTCAAG208750.1335143798984587No Hit
CGTTGTTGAAAGTTGTATTATAATTTTAGAGGTCATACGACCCACGATAC196990.12599280333507729No Hit
CGTTGTTGAAAGTTGTATTTGTGCAAGTTAACGCAAGGTACATTCTGATA194990.12471362364742739No Hit
CGTTGTTGAAAGTTTTAATTTATTAAATCTTTTACTCAGACTACAAAGTT193410.12370307169418396No Hit
CGCTGCTGAAAGTTGTATATAGTTTATAAGGCAACTAAGCCTAATAATGA183000.1170449414199662No Hit
CGTTGTTGAAAGTTTTGATATTTTTATTTGTTGACTAAGTAAATTATTAG179040.1145121656384194No Hit
CGTTGTTGAAAGTTTTAACGATTGTTTAACTAAAAACTCAGACTGCAAAC170100.10879423243462431No Hit
CGTTGTTGAAAGTTTTAACAGTTCGCTTAGAACACTCAGATGTATCGTCG165490.1058457232545913No Hit
CGTTGTTGAAAGTTGTATTATGGTTTATAGGCACAAGGCCCATTGAATGA159200.10182270313693234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGTT11705300.0244.38258
GCTGAAA2057600.0242.052965
CGTTGTT10728850.0241.788271
TGTTGAA9872650.0241.770164
CTGAAAG2063800.0241.675986
GTTGTTG10101050.0241.560622
TGAAAGT11762450.0241.24977
AAAGTTG3511000.0241.189969
TTGTTGA10143950.0241.176653
TGCTGAA2096950.0239.543724
GTTGAAA9792700.0239.056785
TTGAAAG9819300.0238.772236
CGTTGCT2118450.0235.803221
AAAGTTT9029650.0234.320479
GTTGCTG2142650.0234.14872
TGTCGAA136100.0209.717094
TGGAAGT134250.0203.117287
TTGCTGA2507350.0199.529343
GGAAGTT139550.0196.280888
TGACAGT199100.0191.163977
Produced by FastQC (version 0.11.2)