FastQCFastQC Report
Fri 20 May 2016
000000000-AN12D_l01n02.33200000056020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN12D_l01n02.33200000056020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15635020
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGTTGACCA3258882.084346550244259No Hit
GACTTTCCCTCG3227732.0644233266091123No Hit
ACACCTGGTGAT3066061.961020836557932No Hit
CGGAGCTATGGT2943421.88258153811124No Hit
CAAGCATGCCTA2934971.8771770039309192No Hit
CCAGTGTATGCA2830471.8103398652512117No Hit
AATGCCTCAACT2630881.6826841283221896No Hit
AAGAGATGTCGA2576931.6481782562478335No Hit
ATGAGACTCCAC2571171.6444942187474016No Hit
GAATCTTCGAGC2542011.625843778901466No Hit
TCACGGGAGTTG2501751.6000938917890735No Hit
TCCCAGAACAAC2450511.5673213081914832No Hit
AAGGAGCGCCTT2446791.564942033972454No Hit
CCTGAACTAGTT2356151.5069696105281605No Hit
GAATAGAGCCAA2350701.5034838458793145No Hit
ATGTGCACGACT2320011.4838548335723267No Hit
GTCTAATTCCGA2295311.4680569644298505No Hit
AATCAGTCTCGT2222841.4217058884478562No Hit
CAGCTAGAACGC2176451.3920353155928167No Hit
ATCGGCGTTACA2128011.3610535835579358No Hit
CTAGCGAACATC2123971.3584696405888832No Hit
AGTAGAGGGATG2113001.3514533400021234No Hit
CACGGTTGTGAG2099321.3427037509385982No Hit
GACGGAACCCAT2096461.3408745239852586No Hit
CACGCCATAATG2067821.3225566708581122No Hit
GAGTGGTAGAGA2064741.3205867341391313No Hit
TAGGAACTGGCC2035621.3019618778869486No Hit
GCGATATATCGC2029761.2982138814021345No Hit
TCCTCTGTCGAC1976381.2640725755387585No Hit
CTGCTAACGCAA1914401.224430797018488No Hit
ATTCCTGTGAGT1896541.2130077224077744No Hit
TGAGTCACTGGT1889641.2085945524853823No Hit
CTTCGGCAGAAT1878351.2013735831485985No Hit
ACATTCAGCGCA1873451.1982395929138563No Hit
ACACGTAAGCCT1744131.1155278343104136No Hit
GTATGCGCTGTA1738491.1119205475912406No Hit
GAGGAATAGCAG1681481.0754575305947802No Hit
CAGCGGTGACAT1679871.074427790946222No Hit
AGGCATCTTACG1592991.01886022531471No Hit
CTATTTGCGACA1492180.9543831731587169No Hit
ACGTGTACCCAA1451870.928601306554133No Hit
TAGGATTGCTCG1436360.9186812680764079No Hit
GCTGATGAGCTG1434320.9173765047950051No Hit
GACTTGGTATTC1360670.8702707127972973No Hit
TCGAGGACTGCA1352700.8651731817420125No Hit
ACGCGCAGATAC1348040.8621926930697882No Hit
TACAGATGGCTC1338330.8559822756862479No Hit
TTGCGCATACTA1302340.832963437206988No Hit
GTCAATTGACCG1259690.8056849303678537No Hit
GCACGACAACAC1233370.788850925678381No Hit
TTCCTAGGTGAG1223620.7826149247010876No Hit
TTACTGTGCGAT1213470.7761230877862645No Hit
TCCGAATTCACA1176860.752707703603833No Hit
AATCCGTACAGC1103060.7055059731295514No Hit
GGATCGCAGATC1095100.7004148379727049No Hit
GTTGGTCAATCT998590.6386880221451587No Hit
ATCGATCTGTGG967410.6187456108146967No Hit
AGCTGGAAGTCC924970.5916014178427658No Hit
GGTGTCTATTGT837930.5359315178362419No Hit
GTCATATCGTAC772440.4940447789641459No Hit
CCTCGTTCGACT759090.48550625454908275No Hit
GTACGTGGGATC696960.4457685375522385No Hit
ACTGACAGCCAT668260.42741230903446237No Hit
CGAGAAGAGAAC657170.4203192576664436No Hit
TCCAAAGTGTTC496240.31739006409969417No Hit
CGCAGCGGTATA400900.2564115683894232No Hit
CAGGCGTATTGG396870.25383402131880867No Hit
TCTAGCGTAGTG370570.23701280842621242No Hit
AGATGTTCTGCT343730.2198462170179507No Hit
CTAACCTCCGCT277990.1777995806848984No Hit
TTTTTTTTTTTT249490.15957127013588726No Hit
GAACCAAAGGAT185630.11872706270922584No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC650.00126068036.00051161
CCAGCAT953.4248715E-66.00051161
TCCCGGA806.531295E-56.00051161
ACGCGCC751.7493233E-46.00051161
GCGGTGC1202.570414E-85.9998983
TAGGGGG600.00339721215.9998983
GAGCCGG8250.05.9998982
CGTCAGC1405.18412E-105.9998984
AGAGCCT2750.05.9998985
TGGTAGC1601.0913936E-115.9998984
GGCAGTC650.00126222925.99989755
TCCGCAG852.445614E-55.99989753
TAGACGG650.00126222925.99989753
CGGCCGA1054.8323636E-75.99989754
GGGTCAC852.445614E-55.99989752
CGGTTGG1054.8323636E-75.99989753
TACGCGG550.0091623215.99989752
CGAGGTC1101.8159517E-75.99989752
GATGCCG1507.4578566E-115.99989755
AGTTCCG751.751772E-45.99989755
Produced by FastQC (version 0.11.2)