FastQCFastQC Report
Fri 20 May 2016
000000000-AN12D_l01n01.33100000056023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN12D_l01n01.33100000056023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15635020
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC15585559.968359490425978No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC6358864.0670622743047335No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC3232892.067723610203249No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA2882521.8436305166223004No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTT2593191.6585779871084272No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAAATTATTAATCTAATAACA2216131.4174142405957908No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC1904761.2182651509240154No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATTTTGAGAATTGTGCTTA1834571.1733723397859421No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTTGAAACGGGTTGTC1796571.1490679257205938No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAAACAAGGGTGTCCAATTT1786721.142767965758918No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTCACTATGGAGTTGTTG1741131.1136090647789385No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGATAAA1734411.109311021028435No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCTGCCTCCGGGC1660421.0619877684838268No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATGTAAGGATGCTCTTTT1633151.0445461534427203No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAACTGC1588541.016014050509689No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA1584651.01352604601721No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGAATTCGCCCTTCGGGGCTCT1546570.989170464764356No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAACAATCAAACCAATGTAGA1518880.971460221988843No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGTATTCTATTGCCAGCGCTTA1360020.8698549793988111No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTATTGGACGGGTTGTC1357570.8682879842814399No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCTGGCCTCCGGGCC1333040.8525988454124139No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATCTAGGGTGTCCAATTTA1243720.7954706805619691No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTGAAAGGGGTTGTAG1240610.7934815561476736No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAGATTGAAAAGAGTTGTCGC1213690.7762637975519059No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCATAGG1164650.7448983116107303No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTGCCAGCGCTTA1159200.7414125469618843No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGTCTCCTTCGTGA1098060.7023080239104267No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT1037060.6632930434371046No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATTTATGACTGGAGTTGT1009520.6456787391381655No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCCTCGGGGCC958690.6131683873765431No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTCAAGGTGAAGTTGTGC947420.6059602098366359No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGTCCGCCTCTCCG922710.5901559447957214No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTTCCAACGAGCC887400.5675720274102624No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCTGCCTCCGGGC885150.5661329502616562No Hit
GTAGGTGAACCTGCGGAAGGATCATTACATTCAGTAGCTTGCTACTGTTA787260.5035235004496317No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAAACGGGTTGTAG780140.498969620761598No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCTGGA749720.47951329771244294No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTTACCACC717950.45919352837412425No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATTAGGGTGTCCAACTT688850.44058146391881814No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATCAAACAAGTTCATCTTG670520.42885778208150677No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAACCTTTGAGAGTTGAGCTG658240.42100361879933634No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGAAGCTCGGAATGCATGTTCG651400.4166288242675737No Hit
GTAGGTGAACCTGCGGAGGGATCATTACCAAGTACAACCCGTCTCCGGAC648120.4145309695798278No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATGAACTTGACTTGGTTGC635270.40631224008667716No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCCCTCTGGGTCC623460.39875868403110454No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGGCCG615190.3934692760226722No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTGATGCCATACGAGGGGCTG615030.39336694164766023No Hit
GTAGGTGAACCTGCGGGAGGATCATTAGATTGAATTATCATTGTTGCTCG599290.38329979750585547No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCAATTT596940.3817967613728668No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACA587100.3755031973096293No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGATTCGGGCTTCGGCCTACCC575250.3679240576603036No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGAAGTTCGGAATGCATGTTCG574560.3674827406680644No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTAGGGTCTTTTATAGGC533660.34132351605562383No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCGCCC530370.33921926546943976No Hit
GTTGGTGAACCAGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAAACC520690.3330280357812142No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTTGAAAGAGGTATTGTT520520.332919305507764No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGATTGAATTATCATTGTTGCTCG517830.33119880882787484No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCCCGGGC510100.326254779335108No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTACCCGTACTCCCTAAAC499370.31939198031086624No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG494780.31645626292770973No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAATTCGACCCCTTGGGTCAT481340.30786017542670235No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTTTGGGTCC475790.3043104517934739No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATATAAATCGTACTGTTCA462830.2960213674175025No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGTATCTCCGAGTCCGGCGGTC453210.28986851311990647No Hit
GTAGGTGAACCTGCGGAGGGATCATTACCGAGTGGAGGGCGCCCCGGCGC448540.2868816285492439No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTCGTTACGGGGTTGTTTG444310.28417616350986435No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTGAAAGGGGGTTGTA434640.2779913297200771No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATGTTAGGACGCTCTTTT433250.27710229983716045No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATGTAGGGAAGTTGTAGCT403370.25799135530367084No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGAAGCTCGGAACGCGCGTTCG401400.2567313633113357No Hit
GTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACTTTG389340.24901790979480679No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGAGGGCCCTCTGGGTCC383570.2453274763959368No Hit
GTAGGTGAACCTGCGGAAGGATCATTATGAATATATACATTATTGTGGAA368630.23577200412919203No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATAATACTTTGCAGCTTC367310.2349277455353431No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATACAGATGCCTGTCGAGC343860.21992936369764796No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAGAATTATAAATATTTGTGAAAT292280.18693931955315696No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTGACCGAAAGGTCTTATC279370.1786822146693768No Hit
GTAGGTGAACCTGCGGAGGGATCATTACATAAATATGAAGGCGGGCTGGA275260.17605350041125625No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATATCGTGCAAGGTTGAAG269930.17264448654366926No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAAGAAGTATGTGGAGGGAGGGG264420.1691203465041938No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTTGAACGGGTTGTAG252350.1614004970892266No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGC246900.15791473244038062No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAATTGACAGGGTTGT238640.15263172033038655No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGGCCT236330.15115426779115088No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAGTTGCTGAGGGGTTGCTG235670.15073213849422643No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGATGTGCGCAAGCACTTGTCC223990.14326172911835097No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATCTAGGACGTCCAACTTA197080.12605036642102152No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCATTACAC192710.12325535880350648No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTAGACTTCGGTC189790.12138775645953763No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGACCCTCTGGGTCC181650.11618149513080252No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTACTTAACTCATAACCCT177010.11321379825545473No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAACAACATTCAACACTGTGTTT167740.10728480040319743No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAACGAGTTGTAG161470.103274572082415No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATAAATAGGGTGTCCAATT159460.10198899649632683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCTG13374150.0244.539287
GGTGAAC13703350.0244.363534
GTAGGTG13320600.0244.302931
ACCTGCG13402500.0244.24879
TAGGTGA13320250.0243.217732
AACCTGC13444450.0242.877918
AGGTGAA13302400.0242.780673
TGAACCT13523950.0241.199286
ACCAGCG426100.0238.761449
GTGAACC14106950.0237.840325
TTGGTGA467600.0234.337572
TGGTGAA483950.0225.813133
AACCAGC467900.0216.567648
AGGTGGA63650.0215.553823
GTCGACA146900.0212.97824245
GTTGGTG535200.0206.043551
GACCTGC69500.0200.406488
GGTGAAT44350.0189.48144
GAACCAG536000.0189.12087
GGCGAAC68250.0179.666694
Produced by FastQC (version 0.11.2)