FastQCFastQC Report
Tue 19 Apr 2016
000000000-AN0GE_l01n01.331000000522ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AN0GE_l01n01.331000000522ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10912808
Sequences flagged as poor quality0
Sequence length251
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACC25150.044.769817245
CACGGCC2751.207809E-940.053947245
TCACAGC7050.039.92769245
GCAGCCC1250.001393713439.16386245
GAGCACC14200.037.92275245
TCTGCCC2458.7720036E-734.96773245
GTCTTCC9700.034.0665245
CCAGTCC18800.031.898756245
GGCACCC3601.675835E-830.596762245
TACGCCC1650.00543561629.669588245
CTCCAGC37650.029.25587245
GAGCTCC3353.0445335E-729.226759245
CTGCACC2105.0140335E-429.139776245
ATGCCGC54450.028.320972245
CACACGC54350.026.7968245
CCCTGCC2308.5344445E-426.605883245
ACAATCC8400.026.2258245
GCTCACG5851.1459633E-1025.105038245
TCTCGTC6351.2732926E-1125.05562245
TACCACC2000.00352280324.477411245