FastQCFastQC Report
Tue 23 Feb 2016
000000000-AM3NA_l01n03.3330000004a96c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AM3NA_l01n03.3330000004a96c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970890
Sequences flagged as poor quality0
Sequence length251
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG103160.3472360134505148No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG102240.34413929832474444No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG77520.2609319092931748No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA75850.25531069814096113No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG51840.17449316534775774No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA48490.1632170830963112No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA39130.1317113726862994No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG37650.12672970052745136No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA36000.12117580926927622No Hit
CCCAGTTTGGGTCTCAGCTATCGTGTTGATGGAAGTAATAAAGTCACTTT35570.11972843154744875No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG32480.10932750791850254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC234550.0243.073473
TTGGGTC9900.0242.535197
CTGTTCG124000.0241.943362
TTTGCTC217800.0241.181235
TGTTCGA38800.0239.638533
CCTGTTC138900.0237.954331
TTGCTCC235200.0237.718086
TTTGGGT9800.0225.009236
GCTCCCC362350.0224.08268
GGGTCTC11300.0223.327769
GTTTGCT254150.0222.833454
TGCTCCC262650.0221.315957
TGTTTGA44550.0219.982883
CTGTTTG300300.0219.591552
CTCCCCA402000.0214.871389
GGGACTC2750.0213.826959
CTATTCG11700.0211.500842
CTGTTTA32350.0207.895282
TGTTCGC102200.0206.409093
CCTGTAT13350.0204.633851
Produced by FastQC (version 0.11.2)