FastQCFastQC Report
Tue 23 Feb 2016
000000000-AM3NA_l01n02.3320000004a96f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AM3NA_l01n02.3320000004a96f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970890
Sequences flagged as poor quality0
Sequence length12
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAA1108953.732719824698996No Hit
TGAGCCGGAATC883612.9742265785673654No Hit
ACTGACAGCCAT598242.0136726704792167No Hit
CGCCAAATAACC201090.6768678746099653No Hit
CTTACACCAAGT158480.5334428403609693No Hit
ACTATTGTCACG138060.4647092285476742No Hit
GACAGGAGATAG136280.458717757978249No Hit
ACGTGTACCCAA132970.44757631551487936No Hit
GAATGATGAGTG132650.4464991972102636No Hit
CTCGAGAGTACG130510.43929596854814545No Hit
AAAAAAAACAAA128750.4333718178727587No Hit
ATGATGACCCGT125710.4231391939789087No Hit
ACACTAGATCCG121610.40933861570101887No Hit
GCTGATGAGCTG118950.40038506979390015No Hit
AGCAAACACCCG115200.38776258966168387No Hit
GAACTAGTCACC115040.387224030509376No Hit
CAGGAAGGTTAA114700.3860795923107217No Hit
GTATTACGATCC112510.37870806391350736No Hit
GAGTGGTAGAGA112380.3782704846022572No Hit
AAAAAAAAATAA110530.3720433944036972No Hit
TGACCTCCAAGA108540.36534506494686775No Hit
TCGAGGACTGCA107210.3608682919933084No Hit
AAAAAAAAACAA105680.35571832009936416No Hit
ATGGTTGTTGGC105070.3536650633311903No Hit
TTGATGCTATGC100860.33949422563608883No Hit
AAAAAACAAAAA100600.3386190670135885No Hit
CTACCGGATCAA94220.31714402081531123No Hit
ACACCTGGTGAT93990.31636984203386864No Hit
GCAGGATAGATA93580.31498978420607965No Hit
ACGGCATGGCAT92980.31297018738492505No Hit
CGTCCGAAATAC91440.30778655554396156No Hit
AAGAGATGTCGA91290.30728165633867294No Hit
ACCACATACATC91050.3064738176102111No Hit
CACGGTTGTGAG87260.29371669768991787No Hit
ACACGTAAGCCT85030.28621052950462655No Hit
AAAAAAAATAAA85000.28610954966356883No Hit
ATACTTCGCAGG83940.282541595279529No Hit
CCAAGTCTTACA83930.2825079353325098No Hit
GGTCAGCTTAAC83360.2805893183524129No Hit
GTCAATTGACCG82320.2770886838624116No Hit
AGGCTACACGAC81080.2729148504320254No Hit
TAAAAAAAAAAA80540.27109721329298625No Hit
CGTGACAATGTC79060.26611554113413827No Hit
GACTTGGTATTC79010.265947241399042No Hit
GCGATATATCGC78650.2647354833063493No Hit
AAAACAAAAAAA78360.26375934484279123No Hit
AACAAAAAAAAA78100.26288418622029086No Hit
AGGCATCTTACG77400.2605279899289438No Hit
CCTGAACTAGTT75550.25430089973038383No Hit
TGGCACCGATTA74580.2510358848695172No Hit
CTCTACCTCTAC71550.24083692092268646No Hit
AAACAAAAAAAA70660.2378411856379738No Hit
GCACACACGTTA70390.23693236706845422No Hit
TATCGTTGACCA70010.235653289081723No Hit
CAAAAAAAAAAA69620.2343405511479725No Hit
CGGGTTTGACGA69030.2323546142738371No Hit
GCTTCGGTAGAT68400.23023403761162478No Hit
GTATGCGCTGTA68330.22999841798249007No Hit
AATGGAGCATGA67710.22791150126729698No Hit
TAGGATTGCTCG67500.22720464237989288No Hit
TGGCATACGGCA66550.2240069474130648No Hit
ATCCCGAATTTG65650.2209775521813329No Hit
AGCTGGAAGTCC65070.21902527525421678No Hit
ATTCCTGTGAGT63460.21360602378411855No Hit
TAGGAACTGGCC62330.20980244977094406No Hit
AAGATGGATCAG61970.20859069167825128No Hit
TCCAAAGTGTTC59370.199839105453248No Hit
GTCTAATTCCGA56500.19017870065872516No Hit
AATCTTGCTGCA56400.18984210118853273No Hit
CCTCGTTCGACT54620.1838506306191074No Hit
CCATACATAGCT54300.18277351231449163No Hit
GAAGTTGGAAGT53270.17930653777150954No Hit
AATAAAAAAAAA52270.1759405430695852No Hit
TAGCGGATCACG51880.17462780513583473No Hit
TCTGTTGCTCTC51830.17445950540073848No Hit
CTTCGGCAGAAT51790.17432486561266153No Hit
GACTTTCCCTCG51240.17247356852660314No Hit
TGGCAAGACTCT51030.17176670963919902No Hit
GAGGAATAGCAG48600.1635873425135229No Hit
GAACCAAAGGAT48590.16355368256650366No Hit
CAGCTAGAACGC48400.16291414357313802No Hit
CTAGATTTGCCA48310.16261120404996482No Hit
GAGGCTCATCAT48090.16187068521554146No Hit
AGCCGGCACATA47920.16129846611621432No Hit
TCACGGGAGTTG46110.15520601570573128No Hit
CCACCTACTCCA45060.15167172126871073No Hit
GTCTACACACAT44530.1498877440766908No Hit
GTTGGTCAATCT44220.14884428571909428No Hit
GGAGACAAGGGA44000.14810376688467092No Hit
TACTTCGCTCGC43800.14743056794428605No Hit
ACATTCAGCGCA43340.14588221038140087No Hit
CCTCTCGTGATC43310.14578123054034312No Hit
CTTGTGTCGATA43140.145209011441016No Hit
AAAAAATAAAAA42860.14426653292447716No Hit
TACTAATCTGCG42680.1436606538781308No Hit
ACGGGACATGCT42390.14268451541457275No Hit
GTCATATCGTAC41820.14076589843447587No Hit
ACAAAAAAAAAA41610.14005903954707175No Hit
AAAAAAATAAAA40860.1375345435206285No Hit
CGGACTACAACT39970.13453880823591585No Hit
AGCTTGACAGCT39080.13154307295120318No Hit
TAATCCACAGCG38990.13124013342802998No Hit
GACTCGAATCGT38840.13073523422274133No Hit
GTGGGATGTTTC38290.12888393713668295No Hit
ATACCTTCGGTA37960.1277731588850479No Hit
TTACTGTGCGAT36070.1214114288984109No Hit
AGCCTAAGCACG35510.1195264718653333No Hit
GCCTGAATTTAC34930.11757419493821718No Hit
ATTGGGCTAGGC34500.1161268172163897No Hit
GACGGAACCCAT32900.11074122569331075No Hit
CTAACCTCCGCT32310.1087552888191754No Hit
AAGAAAAAAAAA32150.1082167296668675No Hit
TACAGATGGCTC32120.10811574982580976No Hit
AACACAAGGAGT31280.10528831427619333No Hit
AAAAGAAAAAAA30630.10310041771994251No Hit
AAAAAGAAAAAA30270.10188865962724974No Hit
TCGGAGTGTTGT30120.10138376042196111No Hit
GGTCACTGACAG29910.100676901534557No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA550.0091547946.00000051
GGTTGTG8850.06.00000054
TGGCAAG4700.06.00000051
GGCACCA550.0091547946.00000052
CCAAAGG5650.06.00000054
GGACTAC4400.06.00000052
GATGTCG8850.06.00000055
ACCGCGT1101.8109313E-76.00000054
GGATTGA550.0091547946.00000053
GGTTTGA7450.06.00000053
GAGCATG6250.06.00000055
TCACGGG4400.06.00000051
CGGGATT550.0091547946.00000051
TCGTCGA1101.8109313E-76.00000051
GGAAGGT10100.06.00000053
TTGACCG8050.06.00000056
TTGATAC1101.8109313E-76.00000051
GATTTGC5650.06.00000054
AGTTGGA5950.06.00000053
ATTTGCC6250.06.00000055
Produced by FastQC (version 0.11.2)