FastQCFastQC Report
Wed 9 Mar 2016
000000000-AM3N7_l01n03.3330000004bcc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AM3N7_l01n03.3330000004bcc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946405
Sequences flagged as poor quality0
Sequence length26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCAC88833912.788471158822443No Hit
CCTGTTTGCTCCCCACGCTTTCGAGC4967627.151353829786775No Hit
CCTGTTTGCTCCCCACGCTTTCGTGC4563046.568923061641238No Hit
CCTGTTCGCTCCCCACGCTTTCGTCC2745993.953109558109554No Hit
CCTGTTTGCTCCCCACGCTTTCGCGC2046772.946516939337686No Hit
CCTGTTTGATACCCACGCTTTCGTGC1632042.3494742964166355No Hit
CCTGTTCGCTCCCCATGCTTTCGCTC1191461.7152181596091793No Hit
CCTGTTTGCTACCCACGCTTTCGCGC1102461.5870943315283228No Hit
CCTGTTCGCTCCCCACGCTTTCGTGC848781.2218982336906645No Hit
CCTGTTCGCTCCCCACGCTTTCGAGC799211.1505375802303495No Hit
CCTGTTCGCTCCCCACGCTTTCGCTC719831.0362626423308172No Hit
CCTGTTCGCTACCCATGCTTTCGCTC676660.9741153877437322No Hit
CCTGTTTGCTACCCACGCTTTCGAAT410800.5913850401754577No Hit
CCTGTTTGCTACCCACGCTTTCGTGC380260.5474198524272628No Hit
CCTGTTTGCTACCCACGCTTTCGCAC360430.5188727118559888No Hit
CCTGTTTGCTACCCACGCTTTCGAGC356970.5138917180901488No Hit
CCTGTTCGCTCCCCACGCTTTCGCTT351090.5054269078753686No Hit
CCTGTTTGATCCCCACGCTTTCGCAC342850.4935646568260848No Hit
CCTGTTTGCTACCCACGCTTTCGGGC278150.4004229525920242No Hit
CCTGTTCGCTCCCCTAGCTTTCGCAC276730.3983787297170263No Hit
CCTGTTTGCTACCCACGCTTTCGAAC265010.38150669303042367No Hit
CCTGTTCGCTACCCACGCTTTCGAGC186710.268786516190749No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGC128550.1850597539302704No Hit
CCCATTTGCTCCCCTAGCTTTCGTCT127050.1829003635693571No Hit
CCTGTTTGCTCCCCATGCTTTCGTAC110120.15852804436251558No Hit
CCTGTTTGCTCCCCACGCTTTCGTCC97040.13969816041535155No Hit
CCTGTTCGCTCCCCATGCTTTCGCTT93910.13519223252891244No Hit
CCGGTTTGCTCCCCACACTTTCGCGC93330.13435726825602595No Hit
CCCATTCGCTCCCCTAGCTTTCGTCT92730.13349351211166063No Hit
CCTGTTCGCTCCCCACGCTTTCGTGA90320.13002409159845993No Hit
CCTGTTTGCTCCCCACGCTTTCGTTC86090.1239346107806844No Hit
CCTGTTTGATACCCACACTTTCGAGC83530.12024925123139237No Hit
CCGGTTCGCTCCCCACACTTTCGCGC81440.11724050066185315No Hit
CCTGTTTGCTACCCACACTTTCGAGC81120.11677983071819165No Hit
CCTGTTTGCTCCCCTAGCTTTCGCAC76770.11051759867154304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCAC2150.020.00000210
ACACACG4550.020.00000211
TGTTCGA10800.020.0000023
CCCTAGA250.004485717520.012
AACCACG1600.020.011
CCGTGCT250.004485717520.013
CCCAGGC900.020.012
ACCCTAG703.092282E-1120.011
GACCCAC753.6379788E-1220.010
GGCCCAC250.004485717520.010
GCACCCA305.470373E-420.08
GTGTGCT800.020.04
CCACTAG408.261093E-620.011
CCCCCGG2650.020.011
TTGCGGG451.0216372E-620.019
TGGGGGG305.470373E-420.020
GGGCCTC451.0216372E-620.09
CCCCAGG800.020.011
CCACGGT408.261093E-620.013
CGATCCC1950.020.07
Produced by FastQC (version 0.11.2)