FastQCFastQC Report
Wed 9 Mar 2016
000000000-AM3N7_l01n02.3320000004bcc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AM3N7_l01n02.3320000004bcc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946405
Sequences flagged as poor quality0
Sequence length12
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAGAGTACG1969472.835236356071954No Hit
GAATGATGAGTG1781932.565254977214833No Hit
GCAGGATAGATA1743432.5098306246180577No Hit
CGCCAAATAACC1319221.8991406346160353No Hit
ATGGTTGTTGGC1178601.6967049862482824No Hit
CTTACACCAAGT1144161.647125383561713No Hit
TCTACGGAGAGC1129151.6255170840168403No Hit
ATGATGACCCGT1028351.480406051763466No Hit
TATGCACCAGTG936701.3484673007116632No Hit
CGGACTACAACT928821.3371233033489984No Hit
CTCTACCTCTAC887931.2782583221105017No Hit
ACGGCATGGCAT868821.2507476889124662No Hit
ACCACATACATC845511.2171907627038734No Hit
TGGCACCGATTA817751.1772276450912378No Hit
ACTATTGTCACG800631.1525818031053472No Hit
AGCAAACACCCG729341.0499531772190076No Hit
ATTAGTTCGCGT621320.8944482793617706No Hit
ACGATGCGACCA616900.888085275764946No Hit
TAACACCACATC616520.8875382302068481No Hit
CAGGAAGGTTAA604630.8704214626126752No Hit
AATGTCCGTGAC591880.852066644544912No Hit
AGGCTACACGAC535610.7710607141391842No Hit
GTCCGAAACACT531620.7653167357791548No Hit
GACTCGAATCGT530210.7632869088398964No Hit
AAGATGGATCAG529630.7624519445670098No Hit
ATACTTCGCAGG502400.7232518115485637No Hit
CTAGATTTGCCA468390.6742912340987892No Hit
TAAGGTAAGGTG441140.6350623092088642No Hit
AGCCGGCACATA404260.5819700982018757No Hit
TCAACAGCATCG371510.5348234086552685No Hit
TTGATGCTATGC361610.5205714322732407No Hit
GTATTACGATCC338700.4875903434942247No Hit
ACACTAGATCCG325030.46791109933843483No Hit
CCATACATAGCT323750.4660684195637888No Hit
CCTAGTACTGAT296690.4271130174529127No Hit
ATGGACCGAACC287880.4144301980664819No Hit
GGAGACAAGGGA279120.4018193583587482No Hit
TTATGCAGTCGT271080.3902450260242529No Hit
TAGCGGATCACG244910.3525708621941853No Hit
CGGGTTTGACGA243740.350886537712673No Hit
CGTCCGAAATAC243510.35055543119066623No Hit
TAAACCGCGTGT235250.33866438826990364No Hit
GGCCACGTAGTA232360.3345039628412107No Hit
AACACAAGGAGT228800.3293790097179764No Hit
AGTTCCCGAGTA227250.32714763967836596No Hit
GTCAATTGACCG224820.32364942729368645No Hit
TCTAGCGTAGTG220370.31724323588964365No Hit
GACACATTTCTG215730.3105635217065518No Hit
AGAGCCTACGTT209680.3018539805842015No Hit
ATACCTTCGGTA209260.3012493512831458No Hit
CGTGACAATGTC208610.3003136154600833No Hit
AACGAGAACTGA205230.2954477891801587No Hit
GGTCAGCTTAAC197340.2840893958817547No Hit
ATGTCGAGAGAA195940.28207396487823555No Hit
ATCGCTCGAGGA190130.2737099262136314No Hit
ACAAGGAGGTGA179060.25777362535009113No Hit
GTACATACCGGT171490.24687590199534867No Hit
TACTTCGCTCGC165790.2386702186238781No Hit
GTGGGATGTTTC162800.23436583383779092No Hit
GAACCAAAGGAT161920.23309899149272176No Hit
CCAGTGTATGCA154190.22197093316614855No Hit
CACATCTAACAC145770.2098495552735552No Hit
TGACCTCCAAGA137940.19857753758958768No Hit
GCGATATATCGC137650.19816005545314447No Hit
CCATAGGGTTCA136860.19702277652973013No Hit
GTATGCGCTGTA129410.1862978044038607No Hit
CGAGTTGTAGCG128310.1847142514725243No Hit
GCGGCAATTACG105630.15206426921551508No Hit
TCTTCCGCTACT105160.15138766023576225No Hit
TCGAGGACTGCA102770.14794703159404038No Hit
CAGCTAGAACGC101560.1462051233695703No Hit
GCTGATGAGCTG86210.12410736200955746No Hit
TGGCATACGGCA77000.11084870519354975No Hit
CTAGCGAACATC75160.1081998530174961No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG650.00126164196.00000052
AGAAGCT650.00126164196.00000053
GACCATG650.00126164196.00000054
GATGAGG650.00126164196.00000055
ATGAAGG650.00126164196.00000052
GTAAGCC1303.6434358E-96.00000055
TATACGG1303.6434358E-96.00000051
GAGATAG1950.06.00000056
GTGCCAA650.00126164196.00000052
ACGCGAA806.536851E-56.02
GCCCCGA600.00339580776.03
GCCCCAG1054.8276524E-76.04
AGCGTTG550.0091589756.02
AACGTGA550.0091589756.01
GAGTGGT4800.06.01
CAGCGCA1101.81406E-76.06
GACAGGA1750.06.01
CGGCGAG751.750766E-46.05
AATCCGA852.443912E-56.01
CGAACTG2050.06.05
Produced by FastQC (version 0.11.2)