FastQCFastQC Report
Wed 9 Mar 2016
000000000-AM3N7_l01n01.3310000004bcc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AM3N7_l01n01.3310000004bcc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946405
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGA4602746.6260749265267425No Hit
TACGGAGGGTGCGAGCGTTAATCGGA4267356.143249637762267No Hit
TACGTAGGGGGCAAGCGTTATCCGGA2476413.565023922446215No Hit
TACGTAGGTGGCGAGCGTTATCCGGA2265633.261586388930677No Hit
TACGGAGGGTGCAAGCGTTATCCGGA1880832.7076307816777168No Hit
TACGGAGGATGCGAGCGTTATCCGGA1630322.346998195469455No Hit
TACGTAGGGTGCGAGCGTTAATCGGA1519392.187304080312046No Hit
TACGTAGGGGGCTAGCGTTATCCGGA1454512.093903249234676No Hit
TACGTAGGTGGCAAGCGTTGTCCGGA1362501.961446244496254No Hit
TACGTAGGGTGCGAGCGTTGTCCGGA1345181.9365124837955747No Hit
TACGTAGGGAGCAAGCGTTATCCGGA1261861.8165655472147102No Hit
TACGGAGGATACGAGCGTTATCCGGA1204531.7340336476206037No Hit
TACGTAGGTCCCGAGCGTTGTCCGGA991681.4276161554070055No Hit
TACGTAGGGTGCAAGCGTTAATCGGA821781.183029207194225No Hit
TACGAAGGGGGCTAGCGTTGCTCGGA753551.0848057376441482No Hit
TACGTAGGGCGCGAGCGTTGTCCGGA652520.9393635988687674No Hit
TACGAAGGGTGCAAGCGTTACTCGGA570860.821806387620647No Hit
TACAGAGGGTGCGAGCGTTAATCGGA542020.7802885089481537No Hit
TACATAGGGGGCAAGCGTTATCCGGA493310.710165905961429No Hit
TACAGAGGGTGCAAGCGTTAATCGGA442530.6370633442766438No Hit
TACGTAGGTGGCAAGCGTTATCCGGA436170.6279075291463714No Hit
TACGTATGGTGCAAGCGTTATCCGGA432820.623084890673665No Hit
TACGTATGTCACAAGCGTTATCCGGA409730.5898446750513395No Hit
TACGTATGTCACGAGCGTTATCCGGA389110.5601602555566513No Hit
TACGTAGGGCGCAAGCGTTATCCGGA386870.5569355659510207No Hit
TACGTAGGTGGCGAGCGTTGTCCGGA369720.532246536157912No Hit
TACGGAGGGTGCGAGCGTTATTCGGA335790.4834011261940529No Hit
TACGTAGGGCGCAAGCGTTGTCCGGA333570.4802052284599012No Hit
TACGTATGTCGCAAGCGTTATCCGGA330640.4759872192882505No Hit
TACGTATGTCGCGAGCGTTATCCGGA328970.47358309801976706No Hit
TACGGAGGGTGCAAGCGTTACCCGGA192090.2765315296185581No Hit
TACGTAGGGGGCGAGCGTTGTCCGGA188760.2717376830173306No Hit
TACGTAGGGTGCGAGCGTTATCCGGA161940.23312778336420062No Hit
TACGTAGGGTGCTAGCGTTATCCGGA160970.23173137759747667No Hit
GACAGAGGATGCAAGCGTTATCCGGA140260.20191739468113362No Hit
TACGGAGGATCCGAGCGTTATCCGGA110110.15851364842677615No Hit
TACGTAGGGTGCAAGCGTTGTCCGGA109120.15708845078857336No Hit
TACGGAGGGGGCTAGCGTTGTTCGGA93650.13481793819968746No Hit
TACAGAGGATGCAAGCGTTATCCGGA84340.12141532202628554No Hit
TACATAGGTCGCAAGCGTTATCCGGA77650.1117844410166122No Hit
TACGGAGGGTGCAAGCGTTACTCGGA73120.105263082126654No Hit
TCTCGTATGCCGTCTTCTGCTTGAAA71280.10261422995060034Illumina Single End Adapter 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAA5400.020.0000029
GGGTGCT17450.020.0000027
GGGTCCA4300.020.0000027
GGTCCAA4300.020.0000028
GCACGCG1350.020.00000211
GGGCGCG74150.020.0000027
GAGTTAT5400.020.00000215
ACGTATG211050.020.0000022
ACCCGGA20700.020.00000220
ACATAGG73550.020.0000022
AGGTGGA1350.020.0000026
AGGTCAC7950.020.06
GGGGCAC250.004485717520.08
GCGAGAG3450.020.011
TCCGGAG408.261093E-620.01
CTAGATA250.004485717520.03
GAGGATG201800.020.05
GAGGATC13100.020.05
GTGCACG800.020.09
CTAGAGT900.020.012
Produced by FastQC (version 0.11.2)