FastQCFastQC Report
Fri 4 Mar 2016
000000000-AL8FT_l01n03.3330000004af23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AL8FT_l01n03.3330000004af23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984724
Sequences flagged as poor quality0
Sequence length26
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCAC1590848.015421791644581No Hit
CCTGTTTGCTCCCCACGCTTTCGAGC1102645.555633931972405No Hit
CCTGTTTGCTCCCCACGCTTTCGTGC827694.170302772576942No Hit
CCTGTTCGCTCCCCACGCTTTCGTCC539282.7171536193445536No Hit
CCTGTTTGCTCCCCACGCTTTCGCGC474132.3888963906316443No Hit
CCTGTTTGCTACCCACGCTTTCGCGC401152.0211878326659023No Hit
CCTGTTCGCTCCCCATGCTTTCGCTC272581.3733899524568656No Hit
CCTGTTCGCTCCCCACGCTTTCGAGC207151.0437219482406621No Hit
CCTGTTTGATACCCACGCTTTCGTGC204591.0308234293534013No Hit
CCTGTTCGCTACCCATGCTTTCGCTC152820.7699811157621916No Hit
CCTGTTTGATCCCCACGCTTTCGCAC140540.708108532974862No Hit
CCTGTTCGCTCCCCACGCTTTCGCTC131110.6605956294174908No Hit
CCTGTTTGCTCCCCACGCTTTCGCCC116640.5876887668008247No Hit
CCTGTTTCCTCCCCACGCTTTCGCAC110150.5549890060280422No Hit
CCTGTTTGCTCCACACGCTTTCGCAC102550.5166965280814865No Hit
CCTGTTTGCTACCCACGCTTTCGAAC96870.48807793930037624No Hit
CCTGTTTACTCCCCACGCTTTCGCAC90050.4537154788272828No Hit
CCTGTTCGCTCCCCACGCTTTCGTGC88750.4471654497048456No Hit
CCTGTTTGATACCCACACTTTCGAGC85590.43124384045338293No Hit
CCTGTTTCCTCCCCACGCTTTCGAGC84890.4277169016951476No Hit
CCTGTTTGCTACCCACGCTTTCGAGC78920.3976371525713399No Hit
CCTGTTTGCTACCCACGCTTTCGGGC67540.34029920533031294No Hit
CCTGTTTGCTCCACACGCTTTCGAGC64490.32493182931228726No Hit
CCTGTTCGCTCCCCTAGCTTTCGCAC63320.31903680310209376No Hit
CCTGTTCCCTCCCCACGCTTTCGTCC62900.3169206398471526No Hit
CCTGTTTACTCCCCACGCTTTCGAGC61710.3109248439581524No Hit
CCTGTTTCCTCCCCACGCTTTCGTGC59500.29978979445000914No Hit
CCTGTTTGCTCCACACGCTTTCGTGC51950.2617492407004702No Hit
CCTGTTTGCTACCCACGCTTTCGTGC51200.25797037774521797No Hit
CCTGTTCGCTCCCCACGCTTTCGTGA50770.2558038296508734No Hit
CCTGTTTGCTACCCACGCTTTCGCAC50050.25217612121383126No Hit
CCTGTTTACTCCCCACGCTTTCGTGC45250.22799139830021709No Hit
CCTGTTTGCTACCCACGCTTTCGAAT37880.1908577716599386No Hit
CCTGTTTGCTCCCCACGCTTTCGTTC37720.1900516142294848No Hit
CCTGTTCGCTCCACACGCTTTCGTCC37220.18753237225931665No Hit
CCTGTTTCCTCCCCACGCTTTCGCGC35540.1790677192395517No Hit
CCTGTTCACTCCCCACGCTTTCGTCC35250.17760655889685417No Hit
CCTGTTTGCTCCCCACGCTTTCACGC34550.17407962013861877No Hit
CCTGTTTGCTCCCCATGCTTTCGTAC34250.17256807495651788No Hit
CCTGTTCGCTACCCACGCTTTCGAGC34240.1725176901171145No Hit
CCTGTTCCCTCCCCATGCTTTCGCTC33580.16919229071649255No Hit
CCTGTTCGCTCCCCACGCTTTCGCCC30640.15437914793190388No Hit
CCTCTTTGCTCCCCACGCTTTCGCAC29880.1505499001372483No Hit
CCTGTTTGCTCCACACGCTTTCGCGC29850.1503987456190382No Hit
CCTGTTTGCTCCCCACGCTTTCGTCC29550.14888720043693734No Hit
CCTGTTTACTCCCCACGCTTTCGCGC28880.14551141619691202No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGC27420.13815522964402105No Hit
CCTGTTCACTCCCCACGCTTTCGAGC26510.133570209258315No Hit
CCTGTTCGCTCCCCATGCTTTCGCTT24960.12576055915079376No Hit
CCGGTTTGCTCCCCACACTTTCGCGC24800.12495440172033995No Hit
CCTGTTTGATACACACGCTTTCGTGC24110.12147784780150793No Hit
CCTGTTCGCTCCCCACGCTTTCGCTT24070.12127630844389448No Hit
CCTGTTTCATCCCCACGCTTTCGCAC23630.1190593755101465No Hit
CCTGTTTCCTACCCACGCTTTCGCGC23490.11835398775849942No Hit
CCTGTTTGCTCCTCACGCTTTCGCAC23070.11623782450355818No Hit
CCTGTTTGCTCCCCACGCTTTCGACC22990.11583474578833128No Hit
CCTGTTCCCTCCCCACGCTTTCGAGC22400.11286204026353286No Hit
CCTGTTCGCTCCCCACGCTTTCGCGC21360.10762201696558311No Hit
CCTGTTTCATACCCACGCTTTCGTGC21020.10590893242586878No Hit
CCTCTTTGCTCCCCACGCTTTCGAGC21000.10580816274706206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAT1150.024.19961413
TCCCCAT58850.024.19961410
ACCCGCA3100.024.19961211
ACACATG1400.024.19961211
CCCAAGG301.5067126E-424.19961212
GCACGCT4800.024.19961213
CCCTGGC5150.024.19961212
ACCCCCG351.5393107E-524.19961211
CTCAGCT301.5067126E-424.19961213
CCTCACG11050.024.19961211
TCCACAT3450.024.19961210
CCCCCGG1350.024.19961211
CCCCTGG5150.024.19961211
ATACCCG3050.024.1996129
GACCCAA301.5067126E-424.19961210
CCCCATG58250.024.19961211
TCTCAGC351.5393107E-524.19961212
CCGACGC351.5393107E-524.19961212
CTACCCG301.5067126E-424.1996129
CTCCGCA4350.024.1996129
Produced by FastQC (version 0.11.2)