FastQCFastQC Report
Fri 4 Mar 2016
000000000-AL8FT_l01n02.3320000004af26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AL8FT_l01n02.3320000004af26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984724
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGATGAGCTG523862.639460196984568No Hit
ACGTGTACCCAA522122.630693234928383No Hit
GACAGGAGATAG494622.492134926569135No Hit
GAGTGGTAGAGA427842.1556649690334777No Hit
GAACTAGTCACC420882.1205971208087373No Hit
TCGAGGACTGCA416642.0992339489017113No Hit
ATGATGACCCGT376221.8955784280333183No Hit
CACGGTTGTGAG374611.8874664688893772No Hit
GACTTGGTATTC373611.8824279849490408No Hit
ACACCTGGTGAT369881.8636344398515865No Hit
AAGAGATGTCGA345561.741098510422608No Hit
GTCAATTGACCG328211.6536808140577735No Hit
ACACGTAAGCCT322661.6257172281889067No Hit
ACACTAGATCCG317751.6009782720418557No Hit
GCGATATATCGC307371.5486788087411651No Hit
AGGCATCTTACG305021.5368383714813747No Hit
GTATGCGCTGTA289811.4602030307488598No Hit
GCACACACGTTA286651.4442814214973971No Hit
CCTGAACTAGTT279861.4100701155425137No Hit
ATCCCGAATTTG269681.3587783490298904No Hit
TATCGTTGACCA268571.353185631856117No Hit
ACCACATACATC266701.3437636668876882No Hit
ATTCCTGTGAGT250911.2642060054697781No Hit
ATACTTCGCAGG243451.2266189152748694No Hit
TCCAAAGTGTTC243441.226568530435466No Hit
TAGGAACTGGCC241981.219212343882575No Hit
TAGGATTGCTCG234001.1790052420386914No Hit
GAACCAAAGGAT230721.1624790147143884No Hit
AGCAAACACCCG230511.161420933086918No Hit
AGGCTACACGAC226861.1430304667046904No Hit
ATGGTTGTTGGC225081.1340619652908916No Hit
CTTCGGCAGAAT209951.057829703273604No Hit
AGCTGGAAGTCC209641.0562677732520995No Hit
GAAGTTGGAAGT208201.0490123563780154No Hit
GACTTTCCCTCG204811.0319318958202752No Hit
CCTCGTTCGACT200441.0099137210010056No Hit
AATGGAGCATGA199251.0039179251120056No Hit
GTCTAATTCCGA198941.0023559950905012No Hit
GAGGAATAGCAG191930.9670362226687438No Hit
GAGGCTCATCAT190280.958722724167189No Hit
TCACGGGAGTTG176700.8903001122574222No Hit
GTCATATCGTAC174570.8795681414645059No Hit
ACGGGACATGCT173690.87513427559701No Hit
ACATTCAGCGCA161190.8121532263428063No Hit
AATCTTGCTGCA159380.8030335704107976No Hit
GTTGGTCAATCT158480.798498934864495No Hit
CAGCTAGAACGC154350.7776899961909062No Hit
TCTGTTGCTCTC151190.7617683869394435No Hit
TGGCAAGACTCT142830.7196466611982321No Hit
TTACTGTGCGAT141380.7123408594847445No Hit
CCACCTACTCCA140990.7103758507480133No Hit
CCTCTCGTGATC129590.6529371338281796No Hit
CTTGTGTCGATA126050.6351009006793892No Hit
GCTTCGGTAGAT125220.63091895900891No Hit
TACAGATGGCTC118830.5987230466301612No Hit
TACTAATCTGCG116900.5889987726253121No Hit
AGCTTGACAGCT110350.5559967028161095No Hit
TAATCCACAGCG109960.5540316940793784No Hit
TACACGATCTAC105900.5335754492816129No Hit
CAGGAAGGTTAA99900.5033445456395952No Hit
GCCTGAATTTAC99360.5006237643118137No Hit
GACGGAACCCAT98100.49427527454698994No Hit
TGACCTCCAAGA86220.4344180853357948No Hit
GGAGACAAGGGA81700.4116441379254748No Hit
CCATACATAGCT79580.40096255197196184No Hit
ATCGCTCGAGGA75250.3791459165103057No Hit
TCGGAGTGTTGT73570.37068126349054076No Hit
TCAAGCTCAAGC69610.35072886708680906No Hit
TGCAATGTTGCT62960.3172229488835727No Hit
CTGCTAACGCAA61800.31137830751278267No Hit
GTCTACACACAT60840.3065413629300598No Hit
CCATGCGATAAC58330.2938947682398157No Hit
TATGCACCAGTG57600.2902166749633702No Hit
CTATTTGCGACA57440.2894105175329164No Hit
TTGATGCTATGC53850.27132236018710915No Hit
ATTGGGCTAGGC52470.264369252349445No Hit
CGCCAAATAACC51180.25786960806641124No Hit
ATGTGGGACCCA50280.2533349725201086No Hit
GAATGATGAGTG48530.24451762562452006No Hit
CTAACCTCCGCT48370.24371146819406625No Hit
AATGTCCGTGAC47650.24008375975702415No Hit
CGGGTTTGACGA46000.2317702612554693No Hit
ATGAGACTCCAC45260.22804178313962042No Hit
GTCCGAAACACT44140.2223986811264438No Hit
GACTCGAATCGT43840.2208871359443429No Hit
AGATGTTCTGCT43010.21670519427386378No Hit
ACTATTGTCACG41790.21055824386665348No Hit
CTCGAGAGTACG40310.2031012876349558No Hit
TCGTCGATAATC38090.19191585328740923No Hit
CTACCGGATCAA36150.18214119444315682No Hit
ATGGACCGAACC35420.17846310116671135No Hit
AGCCGGCACATA33210.16732805165856815No Hit
ATACCTTCGGTA32150.16198725868181169No Hit
CGACTGTCTTAA30680.15458068728951732No Hit
GCAGGATAGATA29070.1464687281455759No Hit
ACGGCATGGCAT28180.1419844774386766No Hit
CACGCCATAATG26920.1356359876738529No Hit
GGTCACTGACAG26440.13321751538249146No Hit
TAAGGTAAGGTG26100.13150443084277713No Hit
TATCAGGTGTGC25730.1296401917848527No Hit
GTATTACGATCC25440.12817903144215517No Hit
TGGCATACGGCA24120.12152823264091128No Hit
TCAACAGCATCG22840.11507897319728083No Hit
AGAGCCTACGTT22650.11412166124861695No Hit
GTGGGATGTTTC22500.1133658886575665No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAT2350.06.00000054
GTATGCG30900.06.00000051
GGCATGG3050.06.00000053
CGATAAT3650.06.00000055
GCTAGAA16400.06.00000053
GACAGGA49400.06.00000051
TATGCGC31050.06.00000052
GATGTTT2050.06.00000055
CGCCAAA6750.06.00000051
TCAACAG2350.06.00000051
GTGGGAT2050.06.00000051
ATTGTCA3900.06.00000054
CGAATCG4700.06.00000055
GTAAGGT2050.06.00000055
ATGTTTC2050.06.00000056
CAGATGG12500.06.00000053
GATCACG1451.9463187E-106.00000056
GGCTACA24550.06.00000052
CATGGCA3050.06.00000055
TGGGATG2050.06.00000052
Produced by FastQC (version 0.11.2)