FastQCFastQC Report
Fri 4 Mar 2016
000000000-AL8FT_l01n01.3310000004af29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AL8FT_l01n01.3310000004af29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984724
Sequences flagged as poor quality0
Sequence length26
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGA1138435.735961272197041No Hit
TACGGAGGGTGCGAGCGTTAATCGGA993975.008101882176061No Hit
TACGGAGGGTGCAAGCGTTATCCGGA659183.3212678437908747No Hit
TACGTAGGTGGCGAGCGTTATCCGGA578032.9123948720325847No Hit
TACGTAGGGAGCAAGCGTTATCCGGA534732.6942285174160236No Hit
TACGTAGGTGGCAAGCGTTGTCCGGA459742.316392606730205No Hit
TACGGAGGATACGAGCGTTATCCGGA395311.9917630864543383No Hit
TACGTAGGGTGCGAGCGTTGTCCGGA382651.9279758797696809No Hit
TACGTAGGGGGCAAGCGTTATCCGGA374961.8892299382684947No Hit
TACGTAGGTCCCGAGCGTTGTCCGGA357241.7999480028457355No Hit
TACGTAGGGTGCGAGCGTTAATCGGA336791.6969110062658586No Hit
TACGTAGGTGGCAAGCGTTATCCGGA276721.3942492759698577No Hit
TACGTATGTCACAAGCGTTATCCGGA269611.3584256551540668No Hit
TACGGAGGATGCGAGCGTTATCCGGA264851.334442471598066No Hit
TACGTATGTCGCAAGCGTTATCCGGA218791.1023699013061767No Hit
TACGTATGGTGCAAGCGTTATCCGGA216061.0886148401490585No Hit
TACGTAGGGCGCGAGCGTTGTCCGGA215511.0858436739818735No Hit
TACGTAGGGTGCAAGCGTTAATCGGA215221.084382513639176No Hit
TACGTAGGTGTCGAGCGTTATCCGGA196600.9905659426701143No Hit
TACAGAGGGTGCAAGCGTTAATCGGA192370.9692531556024918No Hit
TACGAAGGGTGCAAGCGTTACTCGGA166640.8396129638176391No Hit
TACGTATGTCACGAGCGTTATCCGGA166370.8382525731537483No Hit
TACGGAGGATCCGAGCGTTATCCGGA156230.7871623459987385No Hit
TACGTAGGGGGCTAGCGTTATCCGGA152320.7674618737920235No Hit
TACGTAGGGACCAAGCGTTATCCGGA131670.6634171804240792No Hit
TACGTATGTCGCGAGCGTTATCCGGA126190.6358062884310363No Hit
TACGTAGGTGTCAAGCGTTGTCCGGA114180.5752940963075974No Hit
TACGTAGGGCGCAAGCGTTGTCCGGA111900.5638063529236307No Hit
TACGTAGGTGCCGAGCGTTATCCGGA104570.5268742656409656No Hit
TACGGAGGGTCCGAGCGTTAATCGGA102770.5178049945483604No Hit
TACATAGGGGGCAAGCGTTATCCGGA101770.5127665106080241No Hit
TACAGAGGGTGCGAGCGTTAATCGGA101590.5118595834987636No Hit
TACGGAGGGCGCAAGCGTTAATCGGA101280.5102976534772593No Hit
TACGGAGGGTCCAAGCGTTAATCGGA99950.5035964698366121No Hit
TACGTAGGTCACGAGCGTTGTCCGGA98780.49770144362641855No Hit
TACGGAGGCTACGAGCGTTATCCGGA91030.4586531930888123No Hit
TACGGAGGGCGCGAGCGTTAATCGGA90950.4582501143735854No Hit
TACGGAGGGCGCAAGCGTTATCCGGA86390.435274627605652No Hit
TACGTAGGTCCAGAGCGTTGTCCGGA83450.42046148482106327No Hit
TACGGAGTGTGCAAGCGTTAATCGGA78150.393757519937281No Hit
TACGTAGGTGACGAGCGTTATCCGGA76040.3831263188231714No Hit
TACGTAGGGCGCAAGCGTTATCCGGA72730.3664489369806583No Hit
TACGTAGGTGTCAAGCGTTATCCGGA71330.3593950594641875No Hit
TACGTAGGTCTCGAGCGTTGTCCGGA70160.353500033253994No Hit
TACGGAGAGTGCAAGCGTTAATCGGA67680.34100459308196No Hit
TACGGAGTGTGCGAGCGTTAATCGGA67300.3390899691846322No Hit
TACGGAGGGAGCAAGCGTTAATCGGA64930.32714876224603523No Hit
TACGGAGAGTGCGAGCGTTAATCGGA63320.31903680310209376No Hit
TACGGAGGCTCCGAGCGTTATCCGGA59950.3020571122231605No Hit
TACATAGGTCGCAAGCGTTATCCGGA58630.2954063134219166No Hit
TACGGAGGGAGCGAGCGTTAATCGGA57870.29157706562726105No Hit
TACGTAGGTGGCGAGCGTTGTCCGGA56000.28215510065883215No Hit
TACGGAGGGAGCAAGCGTTATCCGGA53560.2698611998444116No Hit
TACGTAGGTCGCGAGCGTTGTCCGGA51490.25943153808791547No Hit
TACGGAGGGTGCAAGCGTTACTCGGA50190.25288150896547834No Hit
TACGTAGGTGCCAAGCGTTGTCCGGA47950.24159530493912504No Hit
TACGTAGGCGGCAAGCGTTATCCGGA47200.23781644198387283No Hit
TACGTAGGGATCAAGCGTTATCCGGA47170.23766528746566273No Hit
TACGGAGGGTGCAAGCGTTACCCGGA46310.23333219127697355No Hit
TACGTAGGGTCCGAGCGTTGTCCGGA44770.22557292600885565No Hit
TACGTAGGGTGCTAGCGTTATCCGGA43960.22149175401718324No Hit
TACGGAGTGTGCAAGCGTTATCCGGA42580.21453864617951915No Hit
TACGGAGGCTGCGAGCGTTATCCGGA42140.2123217132457712No Hit
TACGTAGGGTGCAAGCGTTGTCCGGA39320.19811318853402288No Hit
TACGGAGGGTGCGAGCGTTATTCGGA38520.19408240138175384No Hit
TACGTAGTGAGCAAGCGTTATCCGGA38310.19302431975428322No Hit
TACGTAGGGAGCGAGCGTTGTCCGGA37220.18753237225931665No Hit
TACGGAGCGTGCGAGCGTTAATCGGA36790.18536582416497208No Hit
TACGTATGTCTCAAGCGTTATCCGGA36510.1839550486616779No Hit
TACGTAGGGTCCAAGCGTTAATCGGA35190.177304249860434No Hit
TACGGAGCGTGCAAGCGTTAATCGGA34610.17438192917503895No Hit
TACGTATGTCTCGAGCGTTATCCGGA34010.17135883881083716No Hit
TACGAAGGGGGCTAGCGTTGCTCGGA33320.16788228489200513No Hit
TACGGAGAGTGCAAGCGTTATCCGGA32520.1638514977397361No Hit
TACGTAGGGCTCGAGCGTTGTCCGGA31160.15699915958087873No Hit
TACGTAGGTGACAAGCGTTGTCCGGA30400.1531699117862232No Hit
TACGTAGGGCGCGAGCGTTAATCGGA30260.15246452403457608No Hit
TACGGAGGCTGCGAGCGTTAATCGGA29890.15060028497665168No Hit
TACGGAGGGTGCACGCGTTAATCGGA29840.15034836077963484No Hit
TACGTAGGGTGCAAGCGTTATCCGGA29750.14989489722500457No Hit
TACGTAGGTGCCAAGCGTTATCCGGA29390.14808104300648353No Hit
TACGTAGGGTGCGAGCGTTATCCGGA28670.1444533345694414No Hit
TACGTAGTGTGCGAGCGTTAATCGGA28460.14339525294197078No Hit
TACGTAGTGTGCGAGCGTTGTCCGGA27410.13810484480461768No Hit
TACGTAGAGAGCAAGCGTTATCCGGA26990.13598868154967644No Hit
TACAGAGGATGCAAGCGTTATCCGGA26810.1350817544404159No Hit
TACGTAGGCGGCGAGCGTTATCCGGA26680.13442675152817218No Hit
TACGTAGGGAGCTAGCGTTATCCGGA26500.13351982441891166No Hit
TACGGAGGCTGCAAGCGTTAATCGGA26420.13311674570368476No Hit
TACGTAGGGCGCTAGCGTTATCCGGA25650.1292371130696258No Hit
TACGGAGGCGACGAGCGTTATCCGGA25500.12848134047857537No Hit
TACGGAGGATGCAAGCGTTATCCGGA25400.12797749208454173No Hit
TACGTAGGGGTCAAGCGTTATCCGGA24290.12238477491076845No Hit
TACGTATGTCACACGCGTTATCCGGA24170.1217801568379281No Hit
TACGTAGGTGCCGAGCGTTGTCCGGA23760.1197143784223902No Hit
TACGTATGTCCCGAGCGTTATCCGGA23470.11825321807969269No Hit
TACGTATGTCCCAAGCGTTATCCGGA22650.11412166124861695No Hit
TACGTATGGCGCAAGCGTTATCCGGA22570.11371858253339004No Hit
TACGTAGTGCGCGAGCGTTGTCCGGA22360.1126605009059194No Hit
TACGTAGGTGTCGAGCGTTGTCCGGA22220.11195511315427234No Hit
TACGGAGGCCACGAGCGTTATCCGGA21890.11029241345396135No Hit
TACGTAGGGCCCGAGCGTTGTCCGGA21820.1099397195781378No Hit
TACGTAGGGGGCGAGCGTTGTCCGGA21040.10600970210467552No Hit
TACGTAGAGTGCGAGCGTTAATCGGA21010.10585854758646543No Hit
TACGTAGGCGTCAAGCGTTATCCGGA20480.10318815109808718No Hit
TACGTAGGGTACGAGCGTTGTCCGGA20420.10288584206166701No Hit
TACGTAGAGTGCGAGCGTTGTCCGGA20180.1016766059159863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC408.256211E-620.0000023
CGGGTGC408.256211E-620.0000026
GGGTGTA408.256211E-620.0000027
CGGAACG2150.020.0000023
GATACGG408.256211E-620.0000023
GTACCGA408.256211E-620.0000028
ATAGGTT800.020.0000024
GGTGTAA408.256211E-620.0000028
ATAGGGG14300.020.0000024
ATAGGAT408.256211E-620.0000024
AGGAGCC1600.020.0000026
ATGTGGC800.020.0000026
GTGGGCT800.020.0000027
ATGTGCA408.256211E-620.0000027
GAGTACA408.256211E-620.0000027
GGACTAG800.020.0000029
TTGTCCT408.256211E-620.00000218
GCTGCGA12550.020.0000028
GTGATGG408.256211E-620.00000215
GTAGGTC82050.020.0000024
Produced by FastQC (version 0.11.2)