FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_96.3510000004f954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_96.3510000004f954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGA29220.636042402826853No Hit
GGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTT19213.568904593639575No Hit
ATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATT17612.438162544169613No Hit
CGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA15110.671378091872793No Hit
GCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCC1077.561837455830389No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA231.6254416961130742Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA40.2826855123674912No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA40.2826855123674912No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA30.21201413427561835No Hit
GTCCACGAGGTCTCTAATTTGTGCGAAGCCGCTCCCGTACGCTGCA30.21201413427561835No Hit
GGAAAAGAGGGAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA30.21201413427561835RNA PCR Primer, Index 17 (96% over 28bp)
GTCCACGAGGTCTCTAATGTCGCGTAATGGCTTAGCGTACGCTGCA30.21201413427561835No Hit
GTCCACGAGGTCTCTCCGAGATGGTAATTCATGTCCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTAGATCGCGTCAACTGAAGCCCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTTATTATCAGACATCGCACGCTGCAGGTCGAC20.1413427561837456No Hit
GTCCACGAGGTCTCTCATCTGATAGCATTGCGGTGCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTAATACGACGAAGGATCGGCCGTACGCTGCAG20.1413427561837456No Hit
GTCCACGAGGTCTCTCAGAACGCGCAATTTGACGACGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTATGATCTCGCCAACGGGCTACGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTGGCTGAATCACAAGCATCCACGTACGCTGCA20.1413427561837456No Hit
GGAAACGAGGGAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA20.1413427561837456RNA PCR Primer, Index 17 (96% over 28bp)
GTCCACGAGGTCTCTAGCCCAACTCGTGTAAAGACGTACGCTGCAG20.1413427561837456No Hit
GTCCACGAGGTCTCTGAGGAGACTGTACTATCATGCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTAGGCTTCACGCACCTTGGATCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTGATGATAGACCATACCTCGCCGTACGCTGCA20.1413427561837456No Hit
GTCCACGAGGTCTCTCACTGCGTGTTAAGGATATGCGTACGCTGCA20.1413427561837456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACT351.0607073E-740.09
CTTTCCC254.4913206E-540.016
TTATGCG208.736756E-440.02
GAGGTCT404.8457878E-940.07
TGGCATT351.0607073E-740.030
TCCTTGC254.4913206E-540.032
GTATGCC254.4913206E-540.02
TCCCATC254.4913206E-540.019
GGCATTC351.0607073E-740.031
CATGACC254.4913206E-540.010
CAGTCGG351.0607073E-740.037
ATCTTGG254.4913206E-540.023
TCAAGCT208.736756E-440.021
AGCTCAA208.736756E-440.024
TGACCTT254.4913206E-540.012
TGCGCCA208.736756E-440.05
CCAAATG208.736756E-440.09
TTACTCA208.736756E-440.017
TTTCCCA254.4913206E-540.017
ATGACCT254.4913206E-540.011
Produced by FastQC (version 0.11.2)