FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_95.3510000004f947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_95.3510000004f947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4564
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGT3106.792287467134093No Hit
AAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCACATAGAAA2575.6310254163014895No Hit
TTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGG2194.798422436459246No Hit
TCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTC2084.557405784399649No Hit
AACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAGGGTT2054.491673970201577No Hit
AGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTG2044.469763365468887No Hit
CTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACGCCC1884.1191936897458366No Hit
GTTACCATGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGA1834.009640666082384No Hit
AATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAAAGTCC1813.9658194566170026No Hit
AAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCA1783.9000876424189306No Hit
AAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATTG1763.856266432953549No Hit
CGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTA1663.6371603856266437No Hit
ATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGA1593.4837861524978084No Hit
CAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAAC1362.9798422436459244No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1192.607361963190184Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA60.13146362839614373No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA60.13146362839614373RNA PCR Primer, Index 41 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGC457.4942363E-1040.02
TTAGAAA200.001128567740.014
TAGAAAT200.001128567740.015
TCCTTTG200.001128567740.023
TAGCGCC200.001128567740.033
CGTCAAC457.4942363E-1040.09
GAGAAGC256.637882E-540.03
GTATGCC401.3063982E-840.021
TTGTTAA457.4942363E-1040.020
AACGCCC457.4942363E-1040.040
AACGTAT457.4942363E-1040.025
AAACCAT200.001128567740.06
TTGCAGT200.001128567740.027
ACTAACG256.637882E-540.036
AGCTCTT256.637882E-540.07
ATCTCGT401.3063982E-840.016
ATCTTGA200.001128567740.023
AGCTCAT200.001128567740.033
CACATAG457.4942363E-1040.037
TTACTTT256.637882E-540.012
Produced by FastQC (version 0.11.2)